Varettas Kerry
South Eastern Area Laboratory Services, Microbiology Department, CSB Level 3, The St. George Public Hospital, Gray St, Kogarah, Sydney, NSW, 2217, Australia,
Cell Tissue Bank. 2013 Dec;14(4):609-14. doi: 10.1007/s10561-012-9361-x. Epub 2013 Jan 13.
Samples of allograft musculoskeletal tissue are cultured by bacteriology laboratories to determine the presence of bacteria and fungi. In Australia, this testing is performed by 6 TGA-licensed clinical bacteriology laboratories with samples received from 10 tissue banks. Culture methods of swab and tissue samples employ a combination of solid agar and/or broth media to enhance micro-organism growth and maximise recovery. All six Australian laboratories receive Amies transport swabs and, except for one laboratory, a corresponding biopsy sample for testing. Three of the 6 laboratories culture at least one allograft sample directly onto solid agar. Only one laboratory did not use a broth culture for any sample received. An international literature review found that a similar combination of musculoskeletal tissue samples were cultured onto solid agar and/or broth media. Although variations of allograft musculoskeletal tissue samples, culture media and methods are used in Australian and international bacteriology laboratories, validation studies and method evaluations have challenged and supported their use in recovering fungi and aerobic and anaerobic bacteria.
同种异体肌肉骨骼组织样本由细菌学实验室进行培养，以确定是否存在细菌和真菌。在澳大利亚，这项检测由6家获得治疗用品管理局（TGA）许可的临床细菌学实验室进行，样本来自10个组织库。拭子和组织样本的培养方法采用固体琼脂和/或肉汤培养基相结合的方式，以促进微生物生长并最大限度地提高回收率。澳大利亚的所有6家实验室都收到了阿氏运送拭子，除了一家实验室外，还收到了相应的活检样本进行检测。6家实验室中有3家将至少一个同种异体移植样本直接培养在固体琼脂上。只有一家实验室对收到的任何样本都未使用肉汤培养。一项国际文献综述发现，类似的肌肉骨骼组织样本组合被培养在固体琼脂和/或肉汤培养基上。尽管澳大利亚和国际细菌学实验室使用的同种异体肌肉骨骼组织样本、培养基和方法存在差异，但验证研究和方法评估对它们在真菌、需氧菌和厌氧菌回收中的应用提出了挑战并提供了支持。
