Varettas Kerry
South Eastern Area Laboratory Services, Sydney, Australia.
Cell Tissue Bank. 2017 Sep;18(3):343-345. doi: 10.1007/s10561-016-9608-z. Epub 2017 Jan 5.
The bioburden screening process of allograft musculoskeletal tissue samples received at the South Eastern Area Laboratory Services includes the routine use of solid agar and cooked meat (CM) broth media. CM has been routinely sub-cultured onto solid agar plates after aerobic incubation at 35 °C. This study will evaluate whether a visual assessment of CM can replace sub-culture by an in vitro inoculation and a prospective study. Eight challenge organisms were serially diluted and inoculated into CM. The average inoculum of 0.5-5.5 CFU produced visible turbidity of CM after 24-h incubation for 7 of the challenge organisms with one organism producing turbidity after 48-h incubation. The prospective study evaluated 222 CM of which 213 were visually clear and no-growth on sub-culture and 9 turbid CM which were culture positive. Broth cultures are an integral part of the bioburden screening process of allograft musculoskeletal tissue and swab samples and visual assessment of CM can replace sub-culture.
东南地区实验室服务中心接收的同种异体肌肉骨骼组织样本的生物负荷筛查过程包括常规使用固体琼脂和熟肉（CM）肉汤培养基。CM在35°C有氧培养后常规转接至固体琼脂平板上。本研究将通过体外接种和前瞻性研究评估CM的目视评估是否可以替代转接培养。将8种挑战微生物进行连续稀释并接种到CM中。0.5 - 5.5 CFU的平均接种量在24小时培养后，7种挑战微生物使CM产生明显浑浊，1种微生物在48小时培养后产生浑浊。前瞻性研究评估了222份CM，其中213份目视清澈且转接培养无生长，9份浑浊的CM培养呈阳性。肉汤培养是同种异体肌肉骨骼组织和拭子样本生物负荷筛查过程的一个组成部分，CM的目视评估可以替代转接培养。
