Varettas Kerry
South Eastern Area Laboratory Services, Microbiology Department, CSB Level 3, St George Hospital, Sydney, Gray St., Kogarah, Sydney, NSW, 2217, Australia,
Cell Tissue Bank. 2014 Dec;15(4):613-8. doi: 10.1007/s10561-014-9435-z. Epub 2014 Mar 6.
Swab and biopsy samples of allograft musculoskeletal tissue are most commonly collected by tissue banks for bacterial and fungal bioburden testing. An in vitro study was performed using the National Committee for Clinical Laboratory Standards standard 'Quality control of microbiological transport systems' (2003) to validate and evaluate the recovery of six challenge organisms from swab and biopsy samples of allograft musculoskeletal tissue. On average, 8.4 to >100 and 7.2 to >100 % of the inoculum was recovered from swab and biopsy samples respectively. A retrospective review of donor episodes was also performed, consisting of paired swab and biopsy samples received in this laboratory during the period 2001-2012. Samples of allograft femoral heads were collected from living donors during hip operations. From the 3,859 donor episodes received, 21 paired swab and biopsy samples each recovered an isolate, 247 swab samples only and 79 biopsy samples only were culture positive. Low numbers of challenge organisms were recovered from inoculated swab and biopsy samples in the in vitro study and validated their use for bioburden testing of allograft musculoskeletal tissue. Skin commensals were the most common group of organisms isolated during a 12-year retrospective review of paired swab and biopsy samples from living donor allograft femoral heads. Paired swab and biopsy samples are a suitable representative sample of allograft musculoskeletal tissue for bioburden testing.
同种异体肌肉骨骼组织的拭子和活检样本通常由组织库采集，用于细菌和真菌生物负荷检测。采用美国国家临床实验室标准委员会的标准《微生物运输系统的质量控制》（2003年）进行了一项体外研究，以验证和评估从同种异体肌肉骨骼组织的拭子和活检样本中回收六种挑战微生物的情况。平均而言，分别从拭子和活检样本中回收了8.4%至>100%以及7.2%至>100%的接种物。还对供体事件进行了回顾性分析，包括本实验室在2001年至2012年期间收到的配对拭子和活检样本。同种异体股骨头样本在髋关节手术期间从活体供体采集。在收到的3859例供体事件中，21对配对的拭子和活检样本均分离出一株菌株，247份仅拭子样本和79份仅活检样本培养呈阳性。在体外研究中，从接种的拭子和活检样本中回收的挑战微生物数量较少，验证了它们用于同种异体肌肉骨骼组织生物负荷检测的适用性。在对活体供体同种异体股骨头的配对拭子和活检样本进行的为期12年的回顾性分析中，皮肤共生菌是最常见的分离菌群。配对的拭子和活检样本是用于生物负荷检测的同种异体肌肉骨骼组织的合适代表性样本。
