I2MC, Université de Toulouse, UPS, Toulouse, France.
J Physiol Biochem. 2013 Sep;69(3):585-93. doi: 10.1007/s13105-012-0229-0. Epub 2013 Jan 13.
Resveratrol is a naturally occurring polyphenol found in many dietary sources and red wine. Recognized as a cancer chemoprevention agent, an anti-inflammatory factor and an antioxidant molecule, resveratrol has been proposed as a potential anti-obesity compound and to be beneficial in diabetes. Most of the studies demonstrating the anti-adipogenic action of resveratrol were performed as long-term treatments on cultured preadipocytes. The aim of this study was to analyse the acute effects of resveratrol on glucose uptake and lipolysis in human mature adipocytes. Samples of subcutaneous abdominal adipose tissue were obtained from overweight humans and immediately digested by liberase. Fat cells were incubated (from 45 min to 4 h) with resveratrol 1 μM-1 mM. Then, glycerol release or hexose uptake was determined. Regarding lipolysis, the significant effects of resveratrol were found at 100 μM, consisting in a facilitation of isoprenaline stimulation and an impairment of insulin antilipolytic action. At 1 and 10 μM, resveratrol only tended to limit glucose uptake. Resveratrol 100 μM did not change basal glucose uptake but impaired its activation by insulin or by benzylamine. This inhibition was not found with other antioxidants. Such impairment of glucose uptake activation in fat cells may led to a reduced availability of glycerol phosphate and then to a decreased triacylglycerol assembly. Therefore, resveratrol increased triacylglycerol breakdown triggered by β-adrenergic activation and impaired lipogenesis. Consequently, our data indicate that resveratrol can be considered as limiting fat accumulation in human fat cells and further support its use for the mitigation of obesity.
白藜芦醇是一种天然存在的多酚，存在于许多饮食来源和红酒中。作为一种癌症化学预防剂、抗炎因子和抗氧化分子，白藜芦醇已被提出作为一种潜在的抗肥胖化合物，并有益于糖尿病。大多数证明白藜芦醇抗脂肪生成作用的研究都是在培养的前体脂肪细胞上进行的长期治疗。本研究旨在分析白藜芦醇对人成熟脂肪细胞葡萄糖摄取和脂肪分解的急性作用。从超重人群中获得皮下腹部脂肪组织样本，并立即用纤维酶消化。将脂肪细胞用 1 μM-1 mM 的白藜芦醇孵育（45 分钟至 4 小时）。然后，测定甘油释放或己糖摄取。关于脂肪分解，发现白藜芦醇在 100 μM 时具有显著作用，表现为促进异丙肾上腺素刺激和损害胰岛素抗脂肪分解作用。在 1 和 10 μM 时，白藜芦醇仅倾向于限制葡萄糖摄取。白藜芦醇 100 μM 不改变基础葡萄糖摄取，但损害其被胰岛素或苄胺激活。其他抗氧化剂没有发现这种抑制作用。脂肪细胞中葡萄糖摄取激活的这种抑制作用可能导致甘油磷酸酯的可用性降低，从而导致三酰基甘油组装减少。因此，白藜芦醇增加了β-肾上腺素能激活引发的三酰基甘油分解，并损害了脂肪生成。因此，我们的数据表明，白藜芦醇可以被认为是限制人脂肪细胞中脂肪积累的物质，并进一步支持其用于减轻肥胖。
