Pramanik B N, Zechman J M, Das P R, Bartner P L
Schering-Plough Research, Bloomfield, New Jersey 07003.
Biomed Environ Mass Spectrom. 1990 Mar;19(3):164-70. doi: 10.1002/bms.1200190312.
The structural analysis of naturally occurring bacterial phospholipids in mixtures by fast atom bombardment (FAB) mass spectrometry are reported. The bacterial strains examined included several genera of actinomycetes, two strains of Escherichia coli, and one strain each of Proteus mirabilis and Pseudomonas aeruginosa. FAB mass spectrometry proved to be a useful tool for the structural identification of phospholipids in mixtures and provided stable pseudo-molecular ions and characteristic fragment ions which permitted the identification of phosphatidylethanolamine and phosphatidyl choline. Information regarding the chain length of the fatty acids, their degree of unsaturation in the chains and the presence of hydroxyl groups was also obtained. The results obtained by FAB mass spectrometry were supported by high-resolution mass spectral data, tandem mass spectrometric studies and FAB mass spectrometry of components which had been separated and partially purified by thin-layer chromatography. Each organism displayed a highly characteristic phospholipid profile suggesting the possible use of FAB mass spectrometry as a method for rapid bacterial detection and identification.
本文报道了用快原子轰击（FAB）质谱法对混合物中天然存在的细菌磷脂进行结构分析。所检测的细菌菌株包括几个放线菌属、两株大肠杆菌，以及奇异变形杆菌和铜绿假单胞菌各一株。FAB质谱法被证明是鉴定混合物中磷脂结构的一种有用工具，它能提供稳定的准分子离子和特征性碎片离子，从而可鉴定磷脂酰乙醇胺和磷脂酰胆碱。同时还获得了有关脂肪酸链长度、链中不饱和程度以及羟基存在情况的信息。FAB质谱法所得结果得到了高分辨质谱数据、串联质谱研究以及经薄层色谱分离和部分纯化后的组分的FAB质谱分析的支持。每种生物体都呈现出高度特征性的磷脂图谱，这表明FAB质谱法有可能作为一种快速检测和鉴定细菌的方法。
