Department of Soil & Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung 402, Taiwan, ROC.
Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, Yunlin, Taiwan, ROC.
Int J Syst Evol Microbiol. 2013 Aug;63(Pt 8):2806-2812. doi: 10.1099/ijs.0.045831-0. Epub 2013 Jan 11.
A Gram-positive, spore-forming, aerobic, rod-shaped, xylanolytic bacterium designated strain CC-Alfalfa-35(T) was isolated from the rhizosphere of Medicago sativa L. in Taiwan. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain CC-Alfalfa-35(T) was affiliated to the genus Cohnella. Strain CC-Alfalfa-35(T) shared 95.3 % pairwise 16S rRNA gene sequence similarity to the type strain of the type species of the genus Cohnella (Cohnella thermotolerans DSM 17683(T)) besides showing a similarity of 97.4-93.6 % with other recognized species of the genus Cohnella. The DNA-DNA hybridization value between CC-Alfalfa-35(T) and Cohnella thailandensis KCTC 22296(T) was 37.7 % ± 1.7 % (reciprocal value, 55.7 % ± 3.0 %). Predominant cellular fatty acids were iso-C16 : 0 and anteiso-C15 : 0. The polar lipid profile constituted diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, lysyl-phosphatidylglycerol, three unidentified phospholipids and three unidentified aminophospholipids. The major respiratory quinone was MK-7 and the DNA G+C content was 58.3 mol%. Strain CC-Alfalfa-35(T) contained meso-diaminopimelic acid as the major diamino acid in the cell-wall peptidoglycan. Based on the polar lipid and fatty acid profiles, which were in line with those of C. thermotolerans DSM 17683(T), coupled with additional distinguishing genotypic, phenotypic and chemotaxonomic features, strain CC-Alfalfa-35(T) is proposed to represent a novel species within the genus Cohnella, for which the name Cohnella formosensis sp. nov. is proposed. The type strain is CC-Alfalfa-35(T) ( = JCM 18405(T) = BCRC 80428(T)).
一株革兰氏阳性、产芽孢、需氧、杆状、木聚糖分解的细菌，被命名为苜蓿 CC-Alfalfa-35(T) 菌株，从台湾紫花苜蓿的根际土壤中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 CC-Alfalfa-35(T) 属于科恩氏菌属。除了与科恩氏菌属的模式种（耐热科恩氏菌 Cohnella thermotolerans DSM 17683(T)）的 16S rRNA 基因序列相似度为 95.3%外，CC-Alfalfa-35(T) 与其他公认的科恩氏菌属物种的相似度为 97.4-93.6%。CC-Alfalfa-35(T) 与泰国科恩氏菌 Cohnella thailandensis KCTC 22296(T) 的 DNA-DNA 杂交值为 37.7%±1.7%（反向值为 55.7%±3.0%）。主要的细胞脂肪酸为 iso-C16:0 和 anteiso-C15:0。极性脂谱由二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、赖氨酸磷酸甘油、三种未鉴定的磷脂和三种未鉴定的氨基磷脂组成。主要的呼吸醌为 MK-7，DNA G+C 含量为 58.3 mol%。CC-Alfalfa-35(T) 细胞壁肽聚糖中的主要二氨基庚二酸为 meso-二氨基庚二酸。基于极性脂和脂肪酸图谱，与耐热科恩氏菌 DSM 17683(T) 的图谱一致，再加上额外的区别基因型、表型和化学分类学特征，CC-Alfalfa-35(T) 被提议代表科恩氏菌属中的一个新种，命名为台湾科恩氏菌 Cohnella formosensis sp. nov.。模式菌株为 CC-Alfalfa-35(T)（=JCM 18405(T)=BCRC 80428(T)）。
