Kämpfer Peter, Glaeser Stefanie P, McInroy John A, Busse Hans-Jürgen, Clermont Dominique, Criscuolo Alexis
Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany.
Division - Entomology and Plant Pathology Dept., Auburn University, Alabama, USA.
Antonie Van Leeuwenhoek. 2024 Dec 24;118(2):41. doi: 10.1007/s10482-024-02051-y.
A Gram-staining-positive, aerobic bacterium, designated strain JJ-181, was isolated from the root surface of soybean. Based on the 16S rRNA gene sequence similarities, strain JJ-181 was grouped into the genus Cohnella, most closely related to Cohnella hashimotonis F6_2S_P_1 (98.85%) and C. ginsengisoli GR21-5 (98.3%). The pairwise average nucleotide identity and digital DNA-DNA hybridisation values of the JJ-181 genome assembly against publicly available Cohnella type strain genomes were below 84% and 28%, respectively. The fatty acid profile from whole cell hydrolysates, the cell wall diaminoacid, the quinone system, the polar lipid profile, and the polyamine pattern supported the allocation of strain JJ-181 to the genus Cohnella. In addition, the results of physiological and biochemical tests also allowed phenotypic differentiation of strain JJ-181 from its closely-related Cohnella species. Functional analysis revealed that strain JJ-181 has different gene clusters related to swarming motility, chemotaxis ability, and endospore and biofilm formations. The gene content also suggests the ability of strain JJ-181 to synthesise biotin and riboflavin, as well as indole-3-acetic acid, an important phytohormone for plant growth. Based on polyphasic analyses, strain JJ-181 can be classified as a new species of the genus Cohnella, for which we propose the name Cohnella rhizoplanae sp. nov., with strain JJ-181 (= LMG 31678 = CIP 112018 = CCM 9031 = DSM 110650) as the type strain.
从大豆根际分离出一株革兰氏染色阳性需氧细菌，命名为JJ - 181菌株。基于16S rRNA基因序列相似性，JJ - 181菌株被归入科奈氏菌属，与哈希莫托科奈氏菌F6_2S_P_1（98.85%）和人参科奈氏菌GR21 - 5（98.3%）关系最为密切。JJ - 181基因组组装与公开的科奈氏菌属模式菌株基因组的成对平均核苷酸同一性和数字DNA - DNA杂交值分别低于84%和28%。全细胞水解产物的脂肪酸谱、细胞壁二氨基酸、醌系统、极性脂质谱和多胺模式支持将JJ - 181菌株归入科奈氏菌属。此外，生理生化测试结果也使JJ - 181菌株与其密切相关的科奈氏菌物种在表型上得以区分。功能分析表明，JJ - 181菌株具有与群体运动、趋化能力以及芽孢和生物膜形成相关的不同基因簇。基因内容还表明JJ - 181菌株具有合成生物素、核黄素以及吲哚 - 3 - 乙酸（一种对植物生长重要的植物激素）的能力。基于多相分析，JJ - 181菌株可被分类为科奈氏菌属的一个新物种，我们将其命名为根际科奈氏菌（Cohnella rhizoplanae sp. nov.），模式菌株为JJ - 181菌株（= LMG 31678 = CIP 112018 = CCM 9031 = DSM 110650）。
