Guimarães-Marques G M, Batista J S, Guimarães H M, Naice-Daou M V, Lima M P, Formiga K M, Santos J M M, Lima C A P, Rafael M S
Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva, Instituto Nacional de Pesquisa da Amazônia, Manaus, AM, Brasil.
Genet Mol Res. 2012 Dec 6;11(4):4198-203. doi: 10.4238/2012.December.6.3.
The Anopheles albitasis complex includes 6 species, and 3 are considered as malaria vectors in Brazil. Twenty-five polymorphic microsatellite DNA loci were isolated and characterized in 24-36 individuals from the neighborhood of Puraquequara, Manaus, Amazonas State, Brazil. The number of estimated alleles ranged from 2 to 10, the observed heterozygosity ranged from 0.182 to 0.897, and the expected heterozygosity ranged from 0.260 to 0.854. Eleven loci showed significant deviation from Hardy-Weinberg equilibrium. Eleven loci were cross-amplified successfully in 5 Anopheles species. These microsatellite loci will be useful in studies investigating population structure and evolutionary genetics in A. albitarsis sensu lato and other A. albitarsis complex species.
白跗按蚊复合体包括6个物种，其中3种在巴西被视为疟疾媒介。在巴西亚马孙州玛瑙斯市普拉凯夸拉附近采集的24至36个个体中，分离并鉴定了25个多态性微卫星DNA位点。估计的等位基因数量在2至10个之间，观察到的杂合度在0.182至0.897之间，预期杂合度在0.260至0.854之间。11个位点显著偏离哈迪-温伯格平衡。11个位点在5种按蚊中成功进行了交叉扩增。这些微卫星位点将有助于研究白跗按蚊复合组及其他白跗按蚊复合体物种的种群结构和进化遗传学。
