Cunha-Machado A S, Scarpassa V M
Laboratório Temático de Biologia Molecular, Coordenação de Biodiversidade, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM, Brasil.
Laboratório de Genética de Populações e Evolução de Mosquitos Vetores de Malária e Dengue, Coordenação de Biodiversidade, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM, Brasil
Genet Mol Res. 2014 Oct 27;13(4):8856-61. doi: 10.4238/2014.October.27.26.
Anopheles nuneztovari sensu lato consists of cryptic species and genetic lineages, one of which is an important human malaria vector in the northern part of South America. Population structure and evolutionary genetics studies may help in the definition and delimitation of the species and lineages within this species complex, which is relevant information for organizations involved in malaria control efforts. In this study, 10 new microsatellite markers were isolated from 2 repeat-enriched genomic libraries of A. nuneztovari s.l. and were characterized in 37-48 mosquitoes of this species. All loci were highly polymorphic and encompassed 5-25 alleles per locus. The observed (HO) and expected (HE) heterozygosities ranged from 0.354 to 0.866 and from 0.613 to 0.932, respectively. Six of the 10 new loci showed significant deviations from the Hardy-Weinberg equilibrium, and no linkage disequilibrium was detected. The loci described in this study were more polymorphic than the 18 previously characterized loci and appear to be promising markers for use in investigating the fine-scale population genetic structure and the boundaries of the cryptic species and lineages within the A. nuneztovari complex.
努内斯按蚊复合种包括隐种和遗传谱系，其中之一是南美洲北部一种重要的人类疟疾媒介。种群结构和进化遗传学研究可能有助于定义和界定该复合种内的物种和谱系，这对于参与疟疾控制工作的组织来说是相关信息。在本研究中，从两个富含重复序列的努内斯按蚊复合种基因组文库中分离出10个新的微卫星标记，并在37至48只该物种的蚊子中进行了特征分析。所有位点均具有高度多态性，每个位点包含5至25个等位基因。观察到的杂合度（HO）和预期杂合度（HE）分别为0.354至0.866和0.613至0.932。10个新位点中有6个显示出与哈迪-温伯格平衡有显著偏差，未检测到连锁不平衡。本研究中描述的位点比之前表征的18个位点具有更高的多态性，似乎是用于研究努内斯按蚊复合种内精细尺度种群遗传结构以及隐种和谱系边界的有前景的标记。
