Shimizu H, Shargill N S, Arase K, York D A, Bray G A
Section of Diabetes and Clinical Nutrition, School of Medicine, University of Southern California, Los Angeles 90033.
Brain Res. 1990 Mar 5;510(2):216-22. doi: 10.1016/0006-8993(90)91370-v.
It has previously been established that norepinephrine (NE) in the central nervous system is involved in feeding and the development of obesity. The present experiments were carried out to investigate the relationship between the uptake of NE by a crude hypothalamic homogenate and NE-mediated sympathetic activity in interscapular brown adipose tissue (IBAT). Sympathetic nervous system activity was assessed by measuring the binding of the purine nucleotide guanosine-5'-diphosphate (GDP) to mitochondria isolated from IBAT. Four situations known to alter food intake and sympathetic activity, namely, corticotropin releasing factor infusion, adrenalectomy, fenfluramine treatment and obesity due to genetic transmission were studied. In each case, [3H]NE uptake by the hypothalamic preparation and GDP binding to IBAT mitochondria were measured. A highly significant negative correlation between the uptake of NE by hypothalamic homogenates and the binding of GDP to IBAT mitochondria was obtained in both lean and obese animals. These findings are discussed with regard to the regulation of food intake and sympathetic nervous system mediated thermogenesis.
先前已经证实，中枢神经系统中的去甲肾上腺素（NE）与进食及肥胖的发展有关。进行本实验是为了研究下丘脑粗匀浆对NE的摄取与肩胛间棕色脂肪组织（IBAT）中NE介导的交感神经活动之间的关系。通过测量嘌呤核苷酸鸟苷-5'-二磷酸（GDP）与从IBAT分离的线粒体的结合来评估交感神经系统的活动。研究了已知会改变食物摄入量和交感神经活动的四种情况，即促肾上腺皮质激素释放因子输注、肾上腺切除术、芬氟拉明治疗以及遗传传递导致的肥胖。在每种情况下，均测量了下丘脑制剂对[3H]NE的摄取以及GDP与IBAT线粒体的结合。在瘦动物和肥胖动物中，下丘脑匀浆对NE的摄取与GDP与IBAT线粒体的结合之间均获得了高度显著的负相关。就食物摄入量的调节和交感神经系统介导的产热作用对这些发现进行了讨论。
