Lupien J R, Tokunaga K, Kemnitz J W, Groos E, Bray G A
Physiol Behav. 1986;38(1):15-20. doi: 10.1016/0031-9384(86)90126-5.
The effects of treatment with fenfluramine or electrolytic lesions in the lateral hypothalamus (LH) on binding of guanosine 5-diphosphate (GDP) by mitochondria from brown adipose tissue have been compared in 4 experiments. In 2 experiments the lesions were lateral to the anterior hypothalamic nucleus and in the other two they were lateral to the ventromedial hypothalamic nucleus. Binding of GDP to mitochondria was significantly increased 18 hours after an electrolytic lesion in either LH site. d,1-Fenfluramine, 20 mg/kg, also increased GDP binding in both acute experiments. In the other 2 experiments GDP binding was measured 11 days after the LH lesion or after 11 daily injections of fenfluramine. When the chronic lesions were lateral to the VMN, there was a transient drop in food intake and body weight. With more anterior lesions, body weight remained significantly lower than in sham-operated rats although food intake returned slowly to control levels. Fenfluramine-treated rats had lower body weights in both chronic experiments even after food intake returned to normal. GDP-binding to mitochondria from interscapular brown adipose tissue was elevated in both of the chronically-treated fenfluramine groups but was only increased in the LH-lesioned rats whose body weight remained below normal.
在4项实验中，比较了用芬氟拉明治疗或在下丘脑外侧区（LH）进行电解损伤对棕色脂肪组织线粒体鸟苷5 - 二磷酸（GDP）结合的影响。在2项实验中，损伤位于下丘脑前核外侧，在另外两项实验中，损伤位于腹内侧下丘脑核外侧。在任何一个LH部位进行电解损伤18小时后，GDP与线粒体的结合显著增加。在两项急性实验中，20mg/kg的消旋芬氟拉明也增加了GDP结合。在另外两项实验中，在LH损伤后11天或每日注射芬氟拉明11天后测量GDP结合。当慢性损伤位于腹内侧核外侧时，食物摄入量和体重出现短暂下降。损伤部位更靠前时，尽管食物摄入量缓慢恢复到对照水平，但体重仍显著低于假手术大鼠。在两项慢性实验中，即使食物摄入量恢复正常，芬氟拉明处理的大鼠体重也较低。在两个长期用芬氟拉明处理的组中，肩胛间棕色脂肪组织线粒体的GDP结合均升高，但仅在体重仍低于正常水平的LH损伤大鼠中增加。
