Willitzki Annika, Hiemann Rico, Peters Vanessa, Sack Ulrich, Schierack Peter, Rödiger Stefan, Anderer Ursula, Conrad Karsten, Bogdanos Dimitrios P, Reinhold Dirk, Roggenbuck Dirk
Institute of Molecular and Clinical Immunology, Otto-von-Guericke University, 39120 Magdeburg, Germany.
Clin Dev Immunol. 2012;2012:284740. doi: 10.1155/2012/284740. Epub 2012 Dec 19.
Antibody assessment is an essential part in the serological diagnosis of autoimmune diseases. However, different diagnostic strategies have been proposed for the work up of sera in particular from patients with systemic autoimmune rheumatic disease (SARD). In general, screening for SARD-associated antibodies by indirect immunofluorescence (IIF) is followed by confirmatory testing covering different assay techniques. Due to lacking automation, standardization, modern data management, and human bias in IIF screening, this two-stage approach has recently been challenged by multiplex techniques particularly in laboratories with high workload. However, detection of antinuclear antibodies by IIF is still recommended to be the gold standard method for antibody screening in sera from patients with suspected SARD. To address the limitations of IIF and to meet the demand for cost-efficient autoantibody screening, automated IIF methods employing novel pattern recognition algorithms for image analysis have been introduced recently. In this respect, the AKLIDES technology has been the first commercially available platform for automated interpretation of cell-based IIF testing and provides multiplexing by addressable microbead immunoassays for confirmatory testing. This paper gives an overview of recently published studies demonstrating the advantages of this new technology for SARD serology.
抗体评估是自身免疫性疾病血清学诊断的重要组成部分。然而，针对特别是系统性自身免疫性风湿病（SARD）患者血清的检测，已经提出了不同的诊断策略。一般来说，通过间接免疫荧光法（IIF）筛查与SARD相关的抗体后，接着采用涵盖不同检测技术的确认性检测。由于IIF筛查缺乏自动化、标准化、现代数据管理以及存在人为偏差，这种两阶段方法最近受到了多重检测技术的挑战，尤其是在工作量大的实验室。然而，IIF检测抗核抗体仍被推荐为疑似SARD患者血清抗体筛查的金标准方法。为了解决IIF的局限性并满足高效自身抗体筛查的需求，最近引入了采用新型模式识别算法进行图像分析的自动化IIF方法。在这方面，AKLIDES技术是首个可用于基于细胞的IIF检测自动化解读的商业平台，并通过可寻址微珠免疫测定法提供多重检测用于确认性检测。本文概述了最近发表的研究，这些研究证明了这项新技术在SARD血清学中的优势。
