do Nascimento Cássio, Sorgini Maíra Balero, Pita Murillo Sucena, Fernandes Flávio Henrique Carriço Nogueira, Calefi Paulo Linares, Watanabe Evandro, Pedrazzi Vinícius
Department of Dental Materials and Prosthodontics, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Gerodontology. 2014 Sep;31(3):227-36. doi: 10.1111/ger.12035. Epub 2013 Jan 15.
The aim of this in vivo study was to evaluate the efficacy of three antimicrobial solutions on the disinfection of toothbrushes after storage in closed containers.
Sixteen healthy subjects were enrolled in this randomized cross-over clinical investigation. The study was conducted in four phases, in which mouthrinses (chlorhexidine gluconate-based or cetilpiridinium-based) and sterile tap water (control group) were used to individually store used toothbrushes in closed containers during 7 days of toothbrushing. Five toothbrushes were used as negative control for bacterial colonisation before contact with oral cavity. Conventional culture and DNA Checkerboard hybridization were used to detect bacterial contamination on the toothbrushes. Subsequently, the number of bacterial species on the bristles was estimated by the DNA Checkerboard method.
One toothbrush presented bacterial contamination in the negative control test. Both culture and DNA Checkerboard showed positive signals of bacterial contamination in the toothbrushes with no differences in the frequency of detection. The control group showed higher total bacterial counts when compared with the mouthrinse groups. Porphyromonas gingivalis had the highest bacterial count followed by Parvimonas micra.
Culture and DNA Checkerboard showed positive signals of bacterial contamination. Mouthrinses that contains 0.12% of chlorhexidine gluconate were more effective in reducing bacterial colonisation on the toothbrushes.
本体内研究旨在评估三种抗菌溶液对封闭容器中储存后的牙刷消毒效果。
16名健康受试者参与了这项随机交叉临床研究。研究分四个阶段进行，在此期间，在为期7天的刷牙过程中，分别使用漱口水（葡萄糖酸洗必泰或西吡氯铵）和无菌自来水（对照组）将用过的牙刷单独储存在封闭容器中。五支牙刷在接触口腔前用作细菌定植的阴性对照。采用传统培养和DNA棋盘杂交法检测牙刷上的细菌污染情况。随后，通过DNA棋盘法估算刷毛上的细菌种类数量。
在阴性对照试验中，有一支牙刷出现细菌污染。培养法和DNA棋盘杂交法均显示牙刷上有细菌污染的阳性信号，检测频率无差异。与漱口水组相比，对照组的细菌总数更高。牙龈卟啉单胞菌的细菌数量最高，其次是微小单胞菌。
培养法和DNA棋盘杂交法均显示有细菌污染的阳性信号。含0.12%葡萄糖酸洗必泰的漱口水在减少牙刷上的细菌定植方面更有效。
