Ren Yong-Qiang, Han Ju-Qiang, Cao Jian-Biao, Li Shao-Xiang, Fan Gong-Ren
Institute of Liver Disease of People's Liberation Army, Beijing Military General Hospital, Beijing, China.
Asian Pac J Cancer Prev. 2012;13(11):5451-4. doi: 10.7314/apjcp.2012.13.11.5451.
The objective of this study was to evaluate the association of MDR1 gene polymorphisms with susceptibility to hepatocellular carcinoma (HCC).
A total of 689 HCC patients and 680 cancer-free subjects were enrolled. Human MDR1 gene polymorphisms were investigated by created restriction site- polymerase chain reaction (CRS-PCR) and DNA sequencing methods. Multiple logistic regression models were applied to estimate the association between MDR1 gene polymorphisms and susceptibility to HCC.
We detected a novel c.4125A>C polymorphism and our findings suggested that this variant was significantly associated with susceptibility to HCC. A significantly increased susceptibility to HCC was noted in the homozygote comparison (CC versus AA: OR=1.621, 95% CI 1.143-2.300, χ2=7.4095, P=0.0065), recessive model (CC versus AC+AA: OR=1.625, 95% CI 1.167-2.264, χ2=8.3544, P=0.0039) and allele contrast (C versus A: OR=1.185, 95% CI 1.011-1.389, χ2=4.4046, P=0.0358). However, no significant increase was observed in the heterozygote comparison (AC versus AA: OR=0.995, 95% CI 0.794-1.248, χ2=0.0017, P=0.9672) and dominant model (CC+AC versus AA: OR=1.106, 95% CI 0.894-1.369, χ2=0.8560, P=0.3549).
These findings suggest that the c.4125A>C polymorphism of the MDR1 gene might contribute to susceptibility to HCC in the Chinese population. Further work will be necessary to clarify the relationship between the c.4125A>C polymorphism and susceptibility to HCC on larger populations of diverse ethnicity.
本研究旨在评估多药耐药基因1（MDR1）基因多态性与肝细胞癌（HCC）易感性之间的关联。
共纳入689例HCC患者和680例无癌受试者。采用创建限制性位点-聚合酶链反应（CRS-PCR）和DNA测序方法研究人类MDR1基因多态性。应用多因素逻辑回归模型评估MDR1基因多态性与HCC易感性之间的关联。
我们检测到一种新的c.4125A>C多态性，我们的研究结果表明该变异与HCC易感性显著相关。在纯合子比较（CC与AA：OR=1.621，95%CI 1.143-2.300，χ2=7.4095，P=0.0065）、隐性模型（CC与AC+AA：OR=1.625，95%CI 1.167-2.264，χ2=8.3544，P=0.0039）和等位基因对比（C与A：OR=1.185，95%CI 1.011-1.389，χ2=4.4046，P=0.0358）中，HCC易感性显著增加。然而，在杂合子比较（AC与AA：OR=0.995，95%CI 0.794-1.248，χ2=0.0017，P=0.9672）和显性模型（CC+AC与AA：OR=1.106，95%CI 0.894-1.369，χ2=0.8560，P=0.3549）中未观察到显著增加。
这些发现表明，MDR1基因的c.4125A>C多态性可能与中国人群的HCC易感性有关。有必要进一步开展工作，以阐明c.4125A>C多态性与不同种族更大人群的HCC易感性之间的关系。
