Sharma Anita, Pandey Arvind, Sardana Sarita, Sehgal Ashok, Sharma Joginder K
Division of Molecular Diagnostics, Institute of Cytology and Preventive Oncology, Noida, India.
Asian Pac J Cancer Prev. 2012;13(11):5647-52. doi: 10.7314/apjcp.2012.13.11.5647.
The glutathione S-transferases (GSTs) are involved in the metabolism of many xenobiotics, including an array of environmental carcinogens, pollutants, and drugs. Genetic polymorphisms in these genes may lead to inter- individual variation in susceptibility to various diseases. In the present study, GSTM1 and GSTT1 polymorphisms were analysed using a multiplex polymerase chain reaction in 500 normal individuals from Delhi. The frequency of individuals with GSTM1 and GSTT1 null genotypes were 168 (33.6%) and 62 (12.4%) respectively, and 54 (10.8%) were having homozygous null genotype for both the genes GSTM1 and GSTT1 simultaneously. The studied population was compared with reported frequencies from other neighbouring state populations, as well as with those from other ethnic groups; Europeans, Blacks, and Asians. The prevalence of homozygous null GSTM1 genotype is significantly higher in Caucasians and Asians as compared to Indian population. The frequency of GSTT1 homozygous null genotypes is also significantly higher in blacks and Asians. We believe that due to large number of individuals in this study, our results are reliable estimates of the frequencies of the GSTM1, GSTT1 in Delhi. It would provide a basic database for future clinical and genetic studies pertaining to susceptibility and inconsistency in the response and/or toxicity to drugs known to be the substrates for GSTs.
谷胱甘肽S-转移酶（GSTs）参与多种外源性物质的代谢，包括一系列环境致癌物、污染物和药物。这些基因的遗传多态性可能导致个体对各种疾病易感性的个体间差异。在本研究中，采用多重聚合酶链反应对来自德里的500名正常个体的GSTM1和GSTT1多态性进行了分析。GSTM1和GSTT1基因缺失基因型个体的频率分别为168例（33.6%）和62例（12.4%），同时有54例（10.8%）个体GSTM1和GSTT1基因均为纯合缺失基因型。将所研究的人群与来自其他邻近邦人群以及其他种族群体（欧洲人、黑人和亚洲人）报告的频率进行了比较。与印度人群相比，白种人和亚洲人中GSTM1纯合缺失基因型的患病率显著更高。GSTT1纯合缺失基因型的频率在黑人和亚洲人中也显著更高。我们认为，由于本研究中的个体数量众多，我们的结果是对德里人群中GSTM1、GSTT1频率的可靠估计。它将为未来有关已知为GSTs底物的药物的易感性、反应和/或毒性的不一致性的临床和遗传学研究提供一个基础数据库。
