Guo Cun-Li, Yang Hai-Chao, Yang Xiu-Hua, Cheng Wen, Dong Tian-Xiu, Zhu Wen-Jing, Xu Zheng, Zhao Liang
The Third Hospital of Harbin Medical University, Harbin, China.
Asian Pac J Cancer Prev. 2012;13(11):5909-13. doi: 10.7314/apjcp.2012.13.11.5909.
We aimed to analyze the phenotype of tumor-infiltrating lymphocytes (TILs) and non-tumor infiltrating lymphocytes (NILs) in HCC and non-tumor tissues, and evaluate relationships between changes in these cells and the prognosis of HCC.
Lymphocytes were isolated from HCC and corresponding non-tumor tissues and tested by flow cytometry. For comparison, clinical parameters were analyzed.
Compared with the non-tumor tissue, tumor tissue had a lower intensity of NK, NKT and CD8+T cell infiltration. TILs had higher intensity of CD4+CD25+Foxp3+regulatory T cell (Treg cells) infiltration compared with that in NILs. The prevalence of Treg cells was associated with fewer CD8 + T lymphocytes in the HCC immune microenvironment. The frequencies of NK cells and CD8+T cells in TILs of HCC patients with metastasis less than 12 months were lower than those without metastasis. However, the frequency of Treg cells was higher than those without metastasis.
These results suggest that the frequencies of CD8+T, NK and NKT cells as well as Treg cells in the tumor tissue of HCC are significantly associated with patient survival, and could be applied as predictive indicators for HCC prognosis.
我们旨在分析肝癌组织及非肿瘤组织中肿瘤浸润淋巴细胞（TILs）和非肿瘤浸润淋巴细胞（NILs）的表型，并评估这些细胞变化与肝癌预后之间的关系。
从肝癌组织及相应的非肿瘤组织中分离淋巴细胞，并用流式细胞术进行检测。为作比较，分析了临床参数。
与非肿瘤组织相比，肿瘤组织中自然杀伤细胞（NK）、自然杀伤T细胞（NKT）和CD8⁺T细胞浸润强度较低。与NILs相比，TILs中CD4⁺CD25⁺Foxp3⁺调节性T细胞（Treg细胞）浸润强度更高。在肝癌免疫微环境中，Treg细胞的存在与CD8⁺T淋巴细胞数量减少有关。转移时间小于12个月的肝癌患者TILs中NK细胞和CD8⁺T细胞的频率低于无转移患者。然而，Treg细胞的频率高于无转移患者。
这些结果表明，肝癌肿瘤组织中CD8⁺T细胞、NK细胞、NKT细胞以及Treg细胞的频率与患者生存显著相关，可作为肝癌预后的预测指标。
