Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Sohngaardsholmsvej 49, DK-9000 Aalborg, Denmark.
Water Res. 2013 Mar 15;47(4):1529-44. doi: 10.1016/j.watres.2012.12.003. Epub 2012 Dec 27.
The enhanced biological phosphorus removal (EBPR) process is increasingly popular as a sustainable method for removal of phosphorus (P) from wastewater. This study consisted of a comprehensive three-year investigation of the identity and population dynamics of polyphosphate-accumulating organisms (PAOs) and glycogen-accumulating organisms (GAOs) in 28 Danish municipal wastewater treatment plants with nutrient removal. Fluorescence in situ hybridization was applied to quantify ten probe-defined populations of PAO and GAO that in total constituted a large fraction (30% on average) of the entire microbial community targeted by the EUBmix probes. Two PAO genera, Accumulibacter and Tetrasphaera, were very abundant in all EBPR plants (average of 3.7% and 27% of all bacteria, respectively), and their abundance was relatively stable in the Danish full-scale plants without clear temporal variations. GAOs were occasionally present in some plants (Competibacter in 11 plants, Defluviicoccus in 6 plants) and were consistent in only a few plants. This shows that these were not core species in the EBPR communities. The total GAO abundance was always lower than that of Accumulibacter. In plants without EBPR design, the abundance of PAO and GAO was significantly lower. Competibacter correlated in general with high fraction of industrial wastewater. In specific plants Accumulibacter correlated with high C/P ratio of the wastewater and Tetrasphaera with high organic loading. Interestingly, the relative microbial composition of the PAO/GAO species was unique to each plant over time, which gives a characteristic plant-specific "fingerprint".
强化生物除磷（EBPR）工艺作为一种从废水中去除磷（P）的可持续方法越来越受欢迎。本研究对丹麦 28 个具有营养去除功能的城市污水处理厂中聚磷菌（PAO）和糖原积累菌（GAO）的身份和种群动态进行了为期三年的综合调查。荧光原位杂交（FISH）技术用于定量分析 10 种探针定义的 PAO 和 GAO 种群，这些种群共构成了 EUBmix 探针靶向的整个微生物群落的很大一部分（平均占 30%）。两种 PAO 属，Accumulibacter 和 Tetrasphaera，在所有 EBPR 工厂中都非常丰富（分别占所有细菌的平均 3.7%和 27%），并且它们的丰度在丹麦的全规模工厂中相对稳定，没有明显的时间变化。GAO 偶尔会出现在一些工厂中（11 个工厂中有 Competibacter，6 个工厂中有 Defluviicoccus），并且只在少数工厂中稳定存在。这表明它们不是 EBPR 群落的核心物种。GAO 的总丰度始终低于 Accumulibacter。在没有 EBPR 设计的工厂中，PAO 和 GAO 的丰度明显较低。Competibacter 通常与工业废水的高比例相关。在特定的工厂中，Accumulibacter 与废水的高 C/P 比相关，而 Tetrasphaera 与高有机负荷相关。有趣的是，PAO/GAO 物种的相对微生物组成随时间在每个工厂中都是独特的，这赋予了每个工厂特有的“指纹”。
