Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, Haryana, India.
J Dairy Res. 2013 Feb;80(1):21-7. doi: 10.1017/S0022029912000684.
Conventional medical therapies for ulcerative colitis (UC) are still limited due to the adverse side effects like dose-dependent diarrhoea and insufficient potency to keep in remission for long-term periods. So, new alternatives that provide more effective and safe therapies for ulcerative colitis are constantly being sought. In the present study, probiotic LaBb Dahi was selected for investigation of its therapeutic effect on DSS-induced colitis model in mice. LaBb Dahi was prepared by co-culturing Dahi culture of Lactococci along with selected strain of Lactobacillus acidophilus LaVK2 and Bifidobacterium bifidum BbVK3 in buffalo milk. Four groups of mice (12 each) were fed for 17 d with buffalo milk (normal control), buffalo milk plus DSS (Colitis control), Dahi plus DSS, and LaBb Dahi plus DSS, respectively, with basal diet. The disease activity scores, weight loss, organ weight, colon length, myeloperoxidase (MPO) and β-glucoronidase activity was assessed, and the histopathological picture of the colon of mice was studied. All colitis control mice evidenced significant increase in MPO, β-glucoronidase activity and showed high disease activity scores along with histological damage to colonic tissue. Feeding with LaBb Dahi offered significant reduction in MPO activity, β-glucoronidase activity and improved disease activity scores. We found significant decline in length of colon, organ weight and body weight in colitis induced controls which were improved significantly by feeding LaBb Dahi. The present study suggests that LaBb Dahi can be used as a potential nutraceutical intervention to combat UC related changes and may offer effective adjunctive treatment for management of UC.
由于常规医学治疗溃疡性结肠炎（UC）的方法存在副作用，如剂量依赖性腹泻和长期缓解效果不足等，因此仍存在局限性。因此，人们一直在寻求新的替代方法，以提供更有效和安全的溃疡性结肠炎治疗方法。在本研究中，选择益生菌 LaBb Dahi 来研究其对 DSS 诱导的小鼠结肠炎模型的治疗作用。LaBb Dahi 通过在水牛乳中共同培养乳球菌的 Dahi 培养物与选定的嗜酸乳杆菌 LaVK2 和双歧杆菌 BbVK3 来制备。四组小鼠（每组 12 只）分别用水牛乳（正常对照组）、水牛乳加 DSS（结肠炎对照组）、Dahi 加 DSS 和 LaBb Dahi 加 DSS 喂养 17 天，同时给予基础饮食。评估疾病活动评分、体重减轻、器官重量、结肠长度、髓过氧化物酶（MPO）和β-葡萄糖醛酸酶活性，并研究小鼠结肠的组织病理学图片。所有结肠炎对照组的 MPO、β-葡萄糖醛酸酶活性均显著增加，疾病活动评分较高，结肠组织存在组织学损伤。用 LaBb Dahi 喂养可显著降低 MPO 活性、β-葡萄糖醛酸酶活性，并改善疾病活动评分。我们发现结肠炎诱导对照组的结肠长度、器官重量和体重显著下降，用 LaBb Dahi 喂养可显著改善这些指标。本研究表明，LaBb Dahi 可用作潜在的营养干预措施来对抗 UC 相关变化，并可能为 UC 的管理提供有效的辅助治疗。
