Department of Neurosurgery, No. 3 People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 280 Mo He Road, Bao Shan District, Shanghai 201900, China.
J Clin Neurosci. 2013 Apr;20(4):543-7. doi: 10.1016/j.jocn.2012.05.033. Epub 2013 Jan 12.
Special AT-rich-sequence-binding protein 1 (SATB1), a new type of gene regulator, has been reported to be expressed in various human cancers and may be associated with malignancy. The aim of this study was to investigate the expression of SATB1 in astrocytoma and to determine its prognostic value for the overall survival of patients with astrocytoma. The expression of SATB1 protein and messenger RNA (mRNA) in human astrocytoma specimens was examined using immunohistochemistry and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). The relationship between SATB1 expression and O-6-methylguanine-DNA methyltransferase (MGMT) promoter methylation status was also investigated. Spearman's correlation coefficient was used to describe the association between SATB1 expression and the clinical parameters of astrocytoma patients. SATB1 protein and mRNA were expressed at significant levels in astrocytoma specimens. SATB1 expression was positively correlated with astrocytoma pathological grade but negatively correlated with the life span of astrocytoma patients. SATB1 expression was also significantly lower in astrocytoma specimens with MGMT promoter methylation than in those without MGMT promoter methylation. Our findings suggest that SATB1 may have an important role as a positive regulator of astrocytoma development and progression and that SATB1 might be a useful molecular marker for predicting the prognosis of patients with astrocytoma and could be a novel target for treating astrocytoma.
特异性 AT 富含序列结合蛋白 1（SATB1）是一种新型的基因调控因子，已被报道在各种人类癌症中表达，并且可能与恶性肿瘤有关。本研究旨在探讨 SATB1 在星形细胞瘤中的表达情况，并确定其对星形细胞瘤患者总生存期的预后价值。使用免疫组织化学和半定量逆转录聚合酶链反应（RT-PCR）检测人类星形细胞瘤标本中 SATB1 蛋白和信使 RNA（mRNA）的表达。还研究了 SATB1 表达与 O-6-甲基鸟嘌呤-DNA 甲基转移酶（MGMT）启动子甲基化状态之间的关系。Spearman 相关系数用于描述 SATB1 表达与星形细胞瘤患者临床参数之间的相关性。SATB1 蛋白和 mRNA 在星形细胞瘤标本中均有显著表达。SATB1 表达与星形细胞瘤病理分级呈正相关，与星形细胞瘤患者的生存期呈负相关。MGMT 启动子甲基化的星形细胞瘤标本中 SATB1 的表达也明显低于未发生 MGMT 启动子甲基化的标本。我们的研究结果表明，SATB1 可能作为星形细胞瘤发生和发展的正向调控因子发挥重要作用，并且 SATB1 可能是预测星形细胞瘤患者预后的有用分子标志物，也可能成为治疗星形细胞瘤的新靶点。
