Martinou J C, Le Van Thai A, Valette A, Weber M J
Laboratoire de Pharmacologie et Toxicologie Fondamentales, Centre National de la Recherche Scientifique, Toulouse, France.
Brain Res Dev Brain Res. 1990 Mar 1;52(1-2):175-81. doi: 10.1016/0165-3806(90)90233-o.
We have studied the effects of transforming growth factor beta 1 (TGF beta 1) on the survival of embryonic motoneurons in culture. For this purpose, E14 rat embryo motoneurons were purified to more than 90% homogeneity by cell sorting and cultured at low density on monolayers of cortex astrocytes. Subnanomolar concentrations of TGF beta 1 (40-500 pM) increased the survival of motoneurons 2-fold after 9-11 days in culture. The increase in choline acetyltransferase (ChAT) activity per culture caused by TGF beta 1 was attributable to its effects on survival. Comparable results were found with motoneurons cultured on lysed astrocytes, suggesting that the effects of the factor are not mediated by non-neuronal cells, but that motoneurons are a target for TGF beta 1.
我们研究了转化生长因子β1（TGFβ1）对培养的胚胎运动神经元存活的影响。为此，通过细胞分选将E14大鼠胚胎运动神经元纯化至同质性超过90%，并以低密度培养在皮质星形胶质细胞单层上。亚纳摩尔浓度的TGFβ1（40 - 500 pM）在培养9 - 11天后使运动神经元的存活率提高了2倍。TGFβ1导致的每培养物中胆碱乙酰转移酶（ChAT）活性的增加归因于其对存活的影响。在裂解的星形胶质细胞上培养的运动神经元也得到了类似的结果，这表明该因子的作用不是由非神经元细胞介导的，而是运动神经元是TGFβ1的作用靶点。
