McManaman J L, Oppenheim R W, Prevette D, Marchetti D
Wagner ALS Research Laboratory, Jerry Lewis Neuromuscular Disease Research Center, Baylor College of Medicine, Houston, Texas 77030.
Neuron. 1990 Jun;4(6):891-8. doi: 10.1016/0896-6273(90)90142-3.
Rat skeletal muscle contains a 22 kd polypeptide that increases the level of choline acetyltransferase (ChAT) activity in cultures of embryonic rat spinal cord neurons and has been purified to homogeneity. The application of this factor, ChAT development factor or CDF, to developing chick embryos during the period of naturally occurring motoneuron cell death significantly increased the survival of motoneurons but did not affect the survival of dorsal root ganglion neurons or sympathetic preganglionic neurons (column of Terni). These results provide the first demonstration that an isolated, skeletal muscle-derived molecule can selectively enhance the survival of motoneurons in vivo and suggest that CDF may function in vivo to regulate the survival and development of motoneurons.
大鼠骨骼肌含有一种22kd的多肽，它能提高胚胎大鼠脊髓神经元培养物中胆碱乙酰转移酶（ChAT）的活性，并且已被纯化至同质。在自然发生运动神经元细胞死亡的时期，将这种因子，即ChAT发育因子或CDF，应用于发育中的鸡胚，可显著提高运动神经元的存活率，但不影响背根神经节神经元或交感神经节前神经元（特尼柱）的存活。这些结果首次证明，一种分离的、骨骼肌来源的分子可以在体内选择性地增强运动神经元的存活，并表明CDF可能在体内发挥作用，调节运动神经元的存活和发育。
