密花角茴香提取物对 HT-29 人结肠癌细胞的自由基清除作用和抗增殖活性。
Free radical scavenging property and antiproliferative activity of Rhodiola imbricata Edgew extracts in HT-29 human colon cancer cells.
机构信息
Bioprospecting Laboratory, Department of Botany, School of Life Sciences, Bharathiar University, Coimbatore - 641 046, Tamil Nadu, India.
出版信息
Asian Pac J Trop Med. 2013 Jan;6(1):11-9. doi: 10.1016/S1995-7645(12)60194-1.
OBJECTIVE
To investigate the in vitro antioxidant and antiproliferative activity of rhizome extracts of Rhodiola imbricata (R. imbricata) in HT-29 human colon cancer cell line.
METHODS
The successively extracted rhizome of R. imbricata using various solvents was analyzed for their total phenolics, tannins and flavonoid contents. In vitro antioxidant activity was evaluated by employing different assays, including DPPH, ABTS radical scavenging assays, FRAP, phosphomolybdenum reduction assay, superoxide anion, hydroxyl radical scavenging activities and metal chelating ability.
RESULTS
Acetone and methanol extracts recorded higher phenolic content and showed comparable antioxidant activity with standard reference. Additionally, they also inhibited the proliferation of HT-29 cells upon treatment at higher concentration (200 μg/mL) (acetone and methanol, 84% and 84%, respectively). On examination acetone extract exhibited antiproliferative activity in a concentration dependent manner whereas, methanol extract showed both dose dependent and time dependent inhibitory activity.
CONCLUSIONS
The results obtained justify the traditional usage of R. imbricata from their promising antioxidant activity.
目的
研究密花角茴香（R. imbricata）根茎提取物对 HT-29 人结肠癌细胞系的体外抗氧化和抗增殖活性。
方法
用不同溶剂对密花角茴香的根茎进行连续提取，分析其总酚、单宁和类黄酮含量。采用 DPPH、ABTS 自由基清除试验、FRAP、磷钼还原试验、超氧阴离子、羟自由基清除活性和金属螯合能力评估体外抗氧化活性。
结果
丙酮和甲醇提取物的酚类含量较高，且具有与标准参考物相当的抗氧化活性。此外，它们在较高浓度（200μg/mL）下也能抑制 HT-29 细胞的增殖（丙酮和甲醇分别为 84%和 84%）。结果表明，在浓度依赖的方式下，丙酮提取物具有抗增殖活性，而甲醇提取物则表现出剂量依赖和时间依赖的抑制活性。
结论
从其具有的抗氧化活性来看，这些结果证实了密花角茴香的传统用途。
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