Visualizing the location and the dynamics of gene expression in living animals through bioluminescence imaging.

Bioluminescence imaging allows for real-time assessment of gene expression in vivo in models where luciferase expression is controlled by promoter elements of gene of interest. It provides a sensitive means of recording temporal and spatial resolution of gene expression. In this Chapter, protocols for the use of the bioluminescence imaging system in localizing and semi-quantitatively measuring gene expression in mice are discussed.