Brown M W, Horn G
University of Bristol, Department of Anatomy, U.K.
Brain Res Dev Brain Res. 1990 Mar 1;52(1-2):294-7. doi: 10.1016/0165-3806(90)90248-w.
Day-old chicks were exposed for 0.5 h to overhead lighting ('primed') and then imprinted by exposing them for 90 min to a rotating red box. The chicks were otherwise maintained in darkness. Immediately after training these chicks (n = 16), together with 16 primed and 16 dark-reared controls were killed. Samples from the left cerebral hemisphere were removed including (i) the intermediate and medial part of the hyperstriatum ventrale (IMHV), a region which is crucial for imprinting, (ii) the posterior neostriatum and (iii) the visual Wulst. The samples were incubated with [35S]methionine, processed for SDS slab gel electrophoresis and autoradiographs prepared. The optical densities of various bands were measured 'blind'. For no band was there a significant interaction (ANOVA) between brain region and experience. Optical density was correlated with approach activity during training, a measure of behaviour which correlates with the amount chicks learn. Only for IMHV were correlations significant: a negative correlation (P less than 0.01) for approximately 50 kDa band and positive correlation (P less than 0.001) for an approximately 80 kDa band. These results suggest that associated with training there is a reduction and an increase in the synthesis of proteins in IMHV.
将一日龄雏鸡置于头顶灯光下0.5小时（“启动”），然后让它们暴露于旋转的红色盒子90分钟进行印记。雏鸡在其他时间保持在黑暗中。训练结束后，立即处死这些雏鸡（n = 16），同时处死16只启动组和16只黑暗饲养的对照组。取左侧大脑半球的样本，包括（i）腹侧上纹状体的中间和内侧部分（IMHV），这是一个对印记至关重要的区域，（ii）后新纹状体，以及（iii）视觉中脑。将样本与[35S]甲硫氨酸一起孵育，进行SDS平板凝胶电泳处理并制备放射自显影片。对各种条带的光密度进行“盲法”测量。对于任何条带，脑区和经验之间均无显著相互作用（方差分析）。光密度与训练期间的接近活动相关，接近活动是一种与雏鸡学习量相关的行为指标。仅IMHV的相关性显著：约50 kDa条带呈负相关（P小于0.01），约80 kDa条带呈正相关（P小于0.001）。这些结果表明，与训练相关的是IMHV中蛋白质合成的减少和增加。
