Miyazaki Y, Arikawa E, Kubota H, Yagi K, Tanabe H
Department of Neurology, Tokyo Metropolitan Neurological Hospital.
Rinsho Shinkeigaku. 1990 Jan;30(1):1-6.
In vitro immunoglobulin (Ig) synthesis was studied using peripheral blood mononuclear cells (PBM) from 12 patients with myasthenia gravis (MG). In cultured PBM, levels of secreted IgG, IgM and IgA measured by ELISA, as well as the number of IgG-, IgM-, and IgA-secreting cells determined by reversed plaque assay, were higher in MG than in controls; this was not dependent upon the stimulation of PBM with pokeweed mitogen (PWM). PBM from 9 of 12 MG patients synthesized anti-acetylcholine receptor (AChR) antibodies in vitro in the presence of PWM, while PBM from 7 controls did not. Patients whose PBM secreted lower levels of each Ig class also secreted fewer or no anti-AChR antibodies. Levels of anti-AChR antibodies secreted in vitro showed a high degree of correlation with titers of sera from patients (r = 0.86). The results suggest that the process of anti-AChR antibody synthesis by PBM in vitro is related to that of Ig synthesis, and that PBM plays, at least in part, an important role in the production of anti-AChR antibodies in MG.
使用12例重症肌无力（MG）患者的外周血单个核细胞（PBM）研究了体外免疫球蛋白（Ig）合成。在培养的PBM中，通过ELISA测定的分泌型IgG、IgM和IgA水平，以及通过反向空斑试验确定的IgG、IgM和IgA分泌细胞数量，MG患者均高于对照组；这并不依赖于用美洲商陆有丝分裂原（PWM）刺激PBM。12例MG患者中有9例的PBM在PWM存在的情况下体外合成抗乙酰胆碱受体（AChR）抗体，而7例对照的PBM则未合成。其PBM分泌各Ig类水平较低的患者分泌的抗AChR抗体也较少或不分泌。体外分泌的抗AChR抗体水平与患者血清滴度高度相关（r = 0.86）。结果表明，PBM体外抗AChR抗体合成过程与Ig合成过程相关，且PBM至少在一定程度上在MG抗AChR抗体产生中起重要作用。
