巴西毁灭腐霉分离株之间的酶变异性
Enzymatic variability among Brazilian Pythium insidiosum isolates.
作者信息
Zanette Régis A, Ferreiro Laerte, Alves Sydney H, Jesus Francielli P K, Lautert Claudia, Spanamberg Andréia, Santurio Janio M
机构信息
Programa de Pós-Graduação em Ciências Veterinárias, Setor de Micologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
出版信息
Rev Iberoam Micol. 2013 Oct-Dec;30(4):264-6. doi: 10.1016/j.riam.2012.12.005. Epub 2013 Jan 11.
BACKGROUND
Pythium insidiosum is an oomycete classified in the kingdom Stramenopila. P. insidiosum hyphae are not able to initiate infection without the secretion of hydrolytic enzymes, which are considered an important factor in microbial virulence.
AIMS
To evaluate the extracellular enzymatic activity of 14 Brazilian P. insidiosum isolates and a standard strain (ATCC 58637) by the API-ZYM System screening method.
METHODS
Zoospores were grown in RPMI 1640 broth, and 65 μL of the liquid phase were inoculated in each cupule of the API-ZYM strips.
RESULTS
Differences in the enzymatic activities were observed among the isolates, although phosphohydrolases and ester hydrolases were conspicuous among all isolates. β-glucosidase was also present in most of the isolates. Enzymatic activities of α-glucosidase and chymotrypsin were not observed, differing from a previous study involving Australian isolates and intracellular enzymes.
CONCLUSIONS
The discrepancy in the enzymatic profile observed among Brazilian P. insidiosum isolates reflects the phenotypic variations found in susceptibility tests.
背景
毁灭腐霉是一种归类于不等鞭毛菌门的卵菌。毁灭腐霉的菌丝若不分泌水解酶就无法引发感染，而水解酶被认为是微生物毒力的一个重要因素。
目的
通过API-ZYM系统筛选法评估14株巴西毁灭腐霉分离株和1株标准菌株（ATCC 58637）的胞外酶活性。
方法
游动孢子在RPMI 1640肉汤中培养，将65 μL液相接种于API-ZYM试条的每个反应杯中。
结果
尽管磷酸水解酶和酯水解酶在所有分离株中都很显著，但各分离株之间的酶活性存在差异。大多数分离株中也存在β-葡萄糖苷酶。未观察到α-葡萄糖苷酶和胰凝乳蛋白酶的酶活性，这与之前一项涉及澳大利亚分离株和细胞内酶的研究不同。
结论
巴西毁灭腐霉分离株之间观察到的酶谱差异反映了药敏试验中发现的表型变异。
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