Centro Nacional de Sanidad Agropecuaria (CENSA), La Habana, Cuba.
Res Vet Sci. 2013 Jun;94(3):781-8. doi: 10.1016/j.rvsc.2012.11.018. Epub 2013 Jan 12.
The emergence of the pandemic H1N1/2009 influenza virus poses a potential global threat for human and animal health. In this study, we carried out pandemic H1N1/2009 influenza virus surveillance in swine herds in Cuba intending to determine whether the virus was circulating among pig populations. As a result we describe, for the first time, the detection of pandemic H1N1/2009 influenza virus in swine herds in Cuba. In addition, phylogenetic analysis and molecular characterization of three viral isolates were performed. Phylogenetic relationships confirmed that all of the eight genes of the three isolates were derived from the pandemic H1N1/2009 virus. The Cuban isolates, formed an independent cluster within the pandemic H1N1/2009 influenza strains. Different molecular markers, previously described in pandemic H1N1/2009 influenza viruses, related with adaptive evolution, viral evasion from the host-immune response, virulence and dissemination were also present in Cuban pandemic H1N1/2009 isolates.
甲型 H1N1/2009 流感病毒大流行的出现对人类和动物健康构成了潜在的全球威胁。在本研究中，我们对古巴猪群中的甲型 H1N1/2009 流感病毒进行了监测，旨在确定该病毒是否在猪群中传播。结果，我们首次描述了在古巴猪群中检测到甲型 H1N1/2009 流感病毒。此外，还对三个病毒分离株进行了系统发育分析和分子特征分析。系统发育关系证实，三个分离株的所有 8 个基因均源自甲型 H1N1/2009 病毒。古巴分离株在甲型 H1N1/2009 流感株内形成了一个独立的聚类。还存在与适应性进化、病毒逃避宿主免疫反应、毒力和传播有关的先前在甲型 H1N1/2009 流感病毒中描述的不同分子标记。
