Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
Biosens Bioelectron. 2013 May 15;43:226-30. doi: 10.1016/j.bios.2012.11.041. Epub 2012 Dec 20.
A novel method of fabricating a free probe type nanoscale biosensor is first demonstrated. A free probe type biosensor based on a single one-dimensional (1D) mesoporous ZnO/Chitosan inorganic-organic hybrid nanostructure is constructed. The as-prepared biosensor exhibited excellent biosensing performance and was stable in solution due to its inorganic-organic hybrid structure. The special mesoporous nanostructure with protuberances favors enzymes loading and enhances electrical communication efficiency. The feasibility of employing a single 1D nanostructure as a separate free nanoprobe for biosensing is demonstrated. This kind of free probe type biosensor will not only provides a new tool for micro-targets detection in microcell and enzymatic studies, but also has the potential to be inserted into single cell or other microorganism for biological studies.
首次展示了一种新型的自由探针式纳米生物传感器的制造方法。构建了基于单个一维（1D）介孔 ZnO/壳聚糖无机-有机杂化纳米结构的自由探针式生物传感器。由于其无机-有机杂化结构，所制备的生物传感器表现出优异的生物传感性能，并且在溶液中稳定。具有突出物的特殊介孔纳米结构有利于酶的负载，并提高了电通信效率。证明了将单个 1D 纳米结构用作独立的自由纳米探针进行生物传感的可行性。这种自由探针式生物传感器不仅为微细胞中微目标的检测和酶研究提供了新工具，而且还有可能插入单细胞或其他微生物中进行生物学研究。
