Institute of Oral Medicine and Department of Stomatology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, No.1 University Rd, Tainan, Taiwan 70101, Republic of China.
Anal Bioanal Chem. 2013 Feb;405(6):1995-2007. doi: 10.1007/s00216-012-6625-z. Epub 2013 Jan 15.
Infrared microspectroscopy is an emerging approach for disease analysis owing to its capability for in situ chemical characterization of pathological processes. Synchrotron-based infrared microspectroscopy (SR-IMS) provides ultra-high spatial resolution for profiling biochemical events associated with disease progression. Spectral alterations were observed in cultured oral cells derived from healthy, precancerous, primary, and metastatic cancers. An innovative wax-physisorption-based kinetic FTIR imaging method for the detection of oral precancer and cancer was demonstrated successfully. The approach is based on determining the residual amount of paraffin wax (C(25)H(52)) or beeswax (C(46)H(92)O(2)) on a sample surface after xylene washing. This amount is used as a signpost of the degree of physisorption that altered during malignant transformation. The results of linear discriminant analysis (LDA) of oral cell lines indicated that the methylene (CH(2)) and methyl group (CH(3)) stretching vibrations in the range of 3,000-2,800 cm(-1) have the highest accuracy rate (89.6 %) to discriminate the healthy keratinocytes (NHOK) from cancer cells. The results of wax-physisorption-based FTIR imaging showed a stronger physisorption with beeswax in oral precancerous and cancer cells as compared with that of NHOK, which showed a strong capability with paraffin wax. The infrared kinetic study of oral cavity tissue showed a consistency in the wax physisorption of the cell lines. On the basis of our findings, these results show the potential use of wax-physisorption-based kinetic FTIR imaging for the early screening of oral cancer lesions and the chemical changes during oral carcinogenesis.
红外微光谱学是一种新兴的疾病分析方法，因为它具有对病理过程进行原位化学表征的能力。基于同步加速器的红外微光谱学（SR-IMS）为分析与疾病进展相关的生化事件提供了超高的空间分辨率。在源自健康、癌前病变、原发性和转移性癌症的培养口腔细胞中观察到了光谱变化。成功地展示了一种基于蜡物理吸附的创新动力学傅里叶变换红外成像方法，用于检测口腔癌前病变和癌症。该方法基于确定二甲苯洗涤后样品表面上的石蜡（C（25）H（52））或蜂蜡（C（46）H（92）O（2））的残留量。该量用作在恶性转化过程中改变的物理吸附程度的标志。线性判别分析（LDA）的口腔细胞系结果表明，在 3000-2800 cm（-1）范围内的亚甲基（CH（2））和甲基（CH（3））伸缩振动具有最高的准确率（89.6％），可将健康角质形成细胞（NHOK）与癌细胞区分开来。基于蜡物理吸附的傅里叶变换红外成像结果表明，与 NHOK 相比，口腔癌前病变和癌细胞具有更强的蜂蜡物理吸附，而 NHOK 具有更强的石蜡物理吸附能力。口腔组织的红外动力学研究表明，细胞系的蜡物理吸附具有一致性。根据我们的发现，这些结果表明基于蜡物理吸附的动力学傅里叶变换红外成像具有用于早期筛查口腔癌病变和口腔癌变过程中化学变化的潜力。
