Hsu Morris M H, Huang Pei-Yu, Lee Yao-Chang, Fang Yuang-Chuen, Chan Michael W Y, Lee Cheng-I
Department of Life Science, National Chung Cheng University, Min-Hsiung, Chia-Yi 62102, Taiwan.
National Synchrotron Radiation Research Center, Hsinchu 30076, Taiwan.
Int J Mol Sci. 2014 Oct 8;15(10):17963-73. doi: 10.3390/ijms151017963.
Ovarian cancer, as well as other cancers, is primarily caused by methylation at cytosines in CpG islands, but the current marker for ovarian cancer is low in sensitivity and failed in early-stage detection. Fourier transform infrared (FT-IR) spectroscopy is powerful in analysis of functional groups within molecules, and infrared microscopy illustrates the location of specific groups within single cells. In this study, we applied HPLC and FT-IR microspectrometry to study normal epithelial ovarian cell line immortalized ovarian surface epithelium (IOSE), two epithelial ovarian cell lines (A2780 and CP70) with distinct properties, and the effect of a cancer drug 5-aza-2'-deoxycytidine (5-aza) without labeling. Our results reveal that inhibition of methylation on cytosine with 5-aza initiates the protein expression. Furthermore, paraffin-adsorption kinetic study allows us to distinguish hypermethylated and hypomethyated cells, and this assay can be a potential diagnosis method for cancer screening.
卵巢癌以及其他癌症主要是由CpG岛中胞嘧啶的甲基化引起的，但目前卵巢癌的标志物敏感性较低，在早期检测中效果不佳。傅里叶变换红外（FT-IR）光谱在分析分子内的官能团方面很强大，而红外显微镜则能显示单细胞内特定基团的位置。在本研究中，我们应用高效液相色谱（HPLC）和傅里叶变换红外显微光谱法来研究永生化卵巢表面上皮（IOSE）正常上皮卵巢细胞系、两种具有不同特性的上皮卵巢细胞系（A2780和CP70）以及未标记的癌症药物5-氮杂-2'-脱氧胞苷（5-aza）的作用。我们的结果表明，用5-aza抑制胞嘧啶甲基化可启动蛋白质表达。此外，石蜡吸附动力学研究使我们能够区分高甲基化和低甲基化细胞，该检测方法可能成为癌症筛查的一种潜在诊断方法。
