甘油和1,2-丙二醇向人血小板的渗透

Permeation of glycerol and propane-1,2-diol into human platelets.

作者信息

Arnaud F G, Pegg D E

机构信息

MRC Medical Cryobiology Group, University Department of Surgery, Cambridge, United Kingdom.

出版信息

Cryobiology. 1990 Apr;27(2):107-18. doi: 10.1016/0011-2240(90)90002-l.

DOI:10.1016/0011-2240(90)90002-l

PMID:2331885

Abstract

The permeability of human platelets to glycerol (GLY) and propane-1,2-diol (propylene glycol, PG) has been determined by measuring the time course of their change in volume following abrupt immersion in solutions of these solutes. A simple light-scattering method, and its calibration to measure mean platelet volume is described. The data are analyzed by means of the Kedem-Katchalsky (K-K) equations, modified to take into account the nonideal behavior of both intracellular and extracellular solutes. The values of the K-K parameters at 2, 21, and 37 degrees C, respectively, were as follows: the hydraulic conductivities (Lp) were 1 x 10(-7), 7 x 10(-7) and 3 x 10(-6) cm.sec-1.atm-1; the solute permeabilities for PG (omega RTPG) were 1.9 x 10(-6), 2.8 x 10(-5), and 1.3 x 10(-4) cm.sec-1; the solute permeabilities for GLY (omega RTGLY), at 21 and 37 degrees C only, were 2.6 x 10(-7) and 1.4 x 10(-6) cm.sec-1. The reflection coefficient (sigma) was 1 throughout. The relevant activation energies were -Lp, 16.5 kcal.mol-1; omega RTPG, 20.5 kcal.mol-1; and omega RTGLY, 17.9 kcal.mol-1. The use of these data is illustrated by computing schedules for the addition and removal of GLY and PG so that the amplitudes of changes in platelet volume are held within predetermined limits.

摘要

通过测量人血小板突然浸入这些溶质溶液后其体积变化的时间过程，已测定了人血小板对甘油（GLY）和1,2 - 丙二醇（丙二醇，PG）的渗透性。描述了一种简单的光散射方法及其用于测量平均血小板体积的校准方法。数据通过Kedem - Katchalsky（K - K）方程进行分析，并进行了修改以考虑细胞内和细胞外溶质的非理想行为。在2℃、21℃和37℃时，K - K参数值分别如下：水力传导率（Lp）为1×10⁻⁷、7×10⁻⁷和3×10⁻⁶cm·sec⁻¹·atm⁻¹；PG的溶质渗透率（ωRTPG）为1.9×10⁻⁶、2.8×10⁻⁵和1.3×10⁻⁴cm·sec⁻¹；仅在21℃和37℃时，GLY的溶质渗透率（ωRTGLY）为2.6×10⁻⁷和1.4×10⁻⁶cm·sec⁻¹。反射系数（σ）始终为1。相关的活化能分别为： - Lp为16.5 kcal·mol⁻¹；ωRTPG为20.5 kcal·mol⁻¹；ωRTGLY为17.9 kcal·mol⁻¹。通过计算添加和去除GLY和PG的时间表来说明这些数据的用途，以便将血小板体积变化的幅度控制在预定范围内。