Department of Obstetrics and Gynecology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, China.
Int J Gynecol Cancer. 2013 Feb;23(2):318-24. doi: 10.1097/IGC.0b013e31827f6f06.
The objective of this study was to analyze the relationship among the protein levels of MCM7, p63, and human papillomavirus (HPV) in different cervical lesion tissues and appraise their predictive value in evaluating severity of cervical disease.
Twelve normal cervix or chronic cervicitis, 42 squamous intraepithelial lesions, and 53 cervical carcinoma tissues were enrolled, and the protein levels of MCM7, p63, and HPV were detected by immunohistochemistry.
The positive examination rates of all the MCM7, p63, and HPV proteins increased gradually and significantly from normal cervix and chronic cervicitis tissues, low-grade squamous intraepithelial lesions, high-grade squamous intraepithelial lesions to cervical carcinomas, respectively. As to predict high-grade squamous intraepithelial lesions and carcinogenesis is concerned, the MCM7 protein had a sensitivity of 94.0%, a specificity of 56.5%, a positive predictive value of 88.8%, and a negative predictive value of 72.2%. The p63 protein had a sensitivity of 78.6%, a specificity of 81.8%, a positive predictive value of 94.3%, and a negative predictive value of 50.0%. Protein level of MCM7 was positively correlated with that of p63 in cervical tissues (r = 0.806, P < 0.01), and the p63 was also positively correlated with histopathologic type (P < 0.05).
Protein levels of MCM7 and p63 were associated significantly with high-grade cervical lesion, and aberrant p63 protein level may distinguish different histopathologic types of cervical carcinoma. They may act as co-predictive index in both HPV-dependent and HPV-independent high-grade cervical lesion with high sensitivity and specificity.
本研究旨在分析不同宫颈病变组织中 MCM7、p63 和人乳头瘤病毒（HPV）蛋白水平之间的关系，并评估其在评估宫颈疾病严重程度中的预测价值。
纳入 12 例正常宫颈或慢性宫颈炎、42 例宫颈鳞状上皮内病变和 53 例宫颈癌组织，采用免疫组织化学法检测 MCM7、p63 和 HPV 蛋白水平。
所有 MCM7、p63 和 HPV 蛋白的阳性检出率均逐渐显著升高，分别从正常宫颈和慢性宫颈炎组织、低级别鳞状上皮内病变、高级别鳞状上皮内病变到宫颈癌组织。就预测高级别鳞状上皮内病变和癌变而言，MCM7 蛋白的敏感性为 94.0%，特异性为 56.5%，阳性预测值为 88.8%，阴性预测值为 72.2%。p63 蛋白的敏感性为 78.6%，特异性为 81.8%，阳性预测值为 94.3%，阴性预测值为 50.0%。宫颈组织中 MCM7 蛋白水平与 p63 蛋白水平呈正相关（r=0.806，P<0.01），且 p63 蛋白水平与组织学类型也呈正相关（P<0.05）。
MCM7 和 p63 蛋白水平与高级别宫颈病变显著相关，异常的 p63 蛋白水平可能区分不同组织学类型的宫颈癌。它们可能作为 HPV 依赖性和 HPV 非依赖性高级别宫颈病变的共同预测指标，具有较高的敏感性和特异性。
