Department of Cell Biology, Embryology and Genetics, Center for Biological Sciences, Federal University of Santa Catarina, Trindade, 88040-900 Florianópolis, SC, Brazil.
Cell Biol Int. 2013 Feb;37(2):181-6. doi: 10.1002/cbin.10015. Epub 2012 Dec 20.
The neural crest (NC) corresponds to a collection of multipotent and oligopotent progenitors endowed with both neural and mesenchymal potentials. The derivatives of the NC at trunk level include neurons and glial cells of the peripheral nervous system. Despite the well-known influence of aflatoxins on the development of cancer, the issue of whether they also influence NC cells has not been yet addressed. In the present work, we have investigated the effects of aflatoxin B(1) on quail NC cells and the concomitant effects of the flavonoid hesperidin associated with this mycotoxin. We show for the first time that aflatoxin B(1) decreases the viability and the total number of glial and neuronal cells/field, although their proportions in relation to the total number of cells were not altered. Therefore, aflatoxin has no effect on NC differentiation. However, this compound was able to reduce NC proliferation and NC survival. Furthermore, the co-administration of hesperidin, a well-known polyphenolic protector of cell death, partially prevented the effect of aflatoxin B(1) . Taken together, our results demonstrate that aflatoxin B(1) is toxic to NC cells, an effect partially prevented by the flavonoid hesperidin. This study may contribute to the understanding of the effects of these compounds during early embryonic development and offer potentially more assertive diets and treatments for pregnant animals.
神经嵴（NC）对应于一群多能和寡能祖细胞，具有神经和间充质潜能。NC 在躯干水平的衍生物包括外周神经系统的神经元和神经胶质细胞。尽管众所周知黄曲霉毒素会影响癌症的发展，但它们是否也会影响 NC 细胞的问题尚未得到解决。在本工作中，我们研究了黄曲霉毒素 B(1)对鹌鹑 NC 细胞的影响，以及与这种真菌毒素相关的类黄酮橙皮苷的伴随作用。我们首次表明，黄曲霉毒素 B(1)降低了神经胶质细胞和神经元细胞/视野的活力和总数，尽管它们与细胞总数的比例没有改变。因此，黄曲霉毒素对 NC 分化没有影响。然而，这种化合物能够减少 NC 的增殖和存活。此外，类黄酮橙皮苷，一种众所周知的细胞死亡保护多酚，可部分预防黄曲霉毒素 B(1)的作用。总之，我们的研究结果表明，黄曲霉毒素 B(1)对 NC 细胞有毒，类黄酮橙皮苷部分预防了这种毒性。这项研究可能有助于理解这些化合物在早期胚胎发育过程中的作用，并为怀孕动物提供更有说服力的饮食和治疗方法。
