Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Cell Tissue Res. 2012 Nov;350(2):305-15. doi: 10.1007/s00441-012-1472-y. Epub 2012 Aug 2.
The neural crest (NC) corresponds to a collection of multipotent and oligopotent progenitors endowed with both neural and mesenchymal potentials. The derivatives of the NC at trunk level include neurons and glial cells of the peripheral nervous system in addition to melanocytes, smooth muscle cells and some endocrine cells. Environmental factors control the fate decisions of NC cells. Despite the well-known influence of flavonoids on the central nervous system, the issue of whether they also influence NC cells has not been yet addressed. Flavonoids are polyphenolic compounds that are integral components of the human diet. The biological activities of these compounds cover a very broad spectrum, from anticancer and antibacterial activities to inhibition of bone reabsorption and modulation of inflammatory response. In the present work, we have investigated the actions of the flavonoids hesperidin, rutin and quercetin on NC cells of quail, in vitro. We show for the first time, that hesperidin and rutin increase the viability of trunk NC cells in culture, without affecting cell differentiation and proliferation. The molecular mechanism of this action is dependent on ERK2 and PI3K pathways. Quercetin had no effect on NC progenitors. Taken together, these results suggest that flavonoids hesperidin and rutin increase NC cell survival, which may be useful against the toxicity of some chemicals during embryonic development.
神经嵴（NC）对应于一群多能和寡能祖细胞，具有神经和间充质潜能。躯干水平的 NC 的衍生物包括外周神经系统的神经元和神经胶质细胞，以及黑色素细胞、平滑肌细胞和一些内分泌细胞。环境因素控制 NC 细胞的命运决定。尽管黄酮类化合物对中枢神经系统的影响众所周知，但它们是否也影响 NC 细胞的问题尚未得到解决。黄酮类化合物是多酚化合物，是人类饮食的组成部分。这些化合物的生物学活性涵盖了非常广泛的范围，从抗癌和抗菌活性到抑制骨吸收和调节炎症反应。在本工作中，我们研究了黄酮类化合物橙皮苷、芦丁和槲皮素对体外鹌鹑躯干 NC 细胞的作用。我们首次表明，橙皮苷和芦丁增加了培养中的躯干 NC 细胞的活力，而不影响细胞分化和增殖。这种作用的分子机制依赖于 ERK2 和 PI3K 途径。槲皮素对 NC 祖细胞没有影响。总之，这些结果表明，黄酮类化合物橙皮苷和芦丁增加了 NC 细胞的存活，这可能有助于对抗胚胎发育过程中某些化学物质的毒性。
