Laboratory of Molecular Genetics, Department of Pathology, Institute of Polish Mother's Memorial Hospital, Lodz, Poland.
Arch Med Sci. 2012 Dec 20;8(6):1065-75. doi: 10.5114/aoms.2012.32417. Epub 2012 Dec 19.
Cigarette smoke and alcohol can generate reactive oxygen species, which may induce DNA double-strand breaks (DSBs), the most serious DNA lesion. In humans, DSBs are repaired mainly by non-homologous end joining and homologous recombination repair (HRR). Several polymorphisms in the DNA repair gene have been extensively studied in the association with various human cancers. In the present work we investigated the association between polymorphisms of two HRR genes, XRCC2 and RAD51, and tobacco- and alcohol-related larynx cancer in a Polish population.
Two polymorphisms of the XRCC2 gene, -41657C > T (rs718282) and 31479G > A (rs3218536), as well as one polymorphism of the RAD51 gene, -135G > C (rs1801320), were investigated by PCR-RFLP in 253 patients with larynx cancer and 253 age- and sex-matched non-cancer controls.
Analysis of the gene-smoking and -drinking interactions revealed a weak association between larynx cancer and the -41657C > T polymorphisms of the XRCC2 gene among the moderate alcohol drinkers. The C allele of the -135G > C polymorphism of RAD51 increased cancer risk in the smoker group. Increased risk was also found for heavy drinkers. Additionally, there were no significant differences between distributions of genotypes in subgroups assigned to different TNM stages and grades.
The results indicated that the -135G > C polymorphism of the RAD51 gene may be associated with smoking- and drinking-related larynx cancer in Poland.
香烟烟雾和酒精会产生活性氧物种，可能会导致 DNA 双链断裂（DSB），这是最严重的 DNA 损伤。在人类中，DSB 主要通过非同源末端连接和同源重组修复（HRR）来修复。几种 DNA 修复基因的多态性已在与各种人类癌症的相关性研究中得到广泛研究。在本工作中，我们研究了 HRR 基因 XRCC2 和 RAD51 的两个多态性与波兰人群中与烟草和酒精相关的喉癌之间的关联。
通过 PCR-RFLP 技术研究了 XRCC2 基因的-41657C>T（rs718282）和 31479G>A（rs3218536）两个多态性以及 RAD51 基因的-135G>C（rs1801320）一个多态性，在 253 例喉癌患者和 253 例年龄和性别匹配的非癌症对照中进行了研究。
基因-吸烟和-饮酒相互作用的分析表明，XRCC2 基因的-41657C>T 多态性与中度饮酒者的喉癌之间存在弱相关性。RAD51 的-135G>C 多态性的 C 等位基因增加了吸烟者的癌症风险。重度饮酒者也发现了风险增加。此外，在分配给不同 TNM 分期和分级的亚组中，基因型的分布没有显著差异。
结果表明，RAD51 基因的-135G>C 多态性可能与波兰的吸烟和饮酒相关的喉癌有关。
