Sakata N, Kawamura K, Takebayashi S
Department of Pathology, School of Medicine, Fukuoka University, Japan.
Exp Mol Pathol. 1990 Apr;52(2):179-91. doi: 10.1016/0014-4800(90)90003-v.
In an attempt to better define the relationship between collagen matrices and vascular smooth muscle cells in vitro, proliferation of smooth muscle cells was observed in the early stages of culture. Cells spread on collagen gels had a longer doubling time and less incorporation of [3H]thymidine into DNA on the first day of culture than did cells grown on a plastic substrate. Cells on collagen gels were more elongated than were those on the plastic substrate and showed a "hills and valleys" arrangement from the first day in culture on the collagen type III gel. All cells were identified as smooth muscle having definite microfilaments, dense bodies, and pinocytotic vesicles. They had a distinct actin filament running from end to end when labeled with nitrobenzoxadiazole-phallacidin. Cells on the collagen gels had a larger number of actin filaments traveling parallel to the direction of the major axis of their cytoplasm than did those on the glass substrate. Therefore, cultured smooth muscle cells in the more physiological environment for cells in vitro, i.e., on collagen gels, show a suppression of cellular proliferation and an enhancement of differentiation in the early stages of culture. The effects of collagens on the differentiation of cells vary with the collagen phenotype.
为了更好地确定体外胶原蛋白基质与血管平滑肌细胞之间的关系，在培养早期观察了平滑肌细胞的增殖情况。与在塑料基质上生长的细胞相比，铺展在胶原蛋白凝胶上的细胞在培养第一天的倍增时间更长，且[3H]胸腺嘧啶核苷掺入DNA的量更少。在胶原蛋白凝胶上的细胞比在塑料基质上的细胞更细长，并且从在III型胶原蛋白凝胶上培养的第一天起就呈现出“丘陵和山谷”的排列。所有细胞均被鉴定为具有明确微丝、致密体和吞饮小泡的平滑肌细胞。用硝基苯并恶二唑-鬼笔环肽标记时，它们有一条从一端延伸到另一端的明显肌动蛋白丝。与在玻璃基质上的细胞相比，在胶原蛋白凝胶上的细胞有更多平行于其细胞质主轴方向排列的肌动蛋白丝。因此，在体外对细胞而言更具生理性的环境中，即在胶原蛋白凝胶上培养的平滑肌细胞，在培养早期表现出细胞增殖的抑制和分化的增强。胶原蛋白对细胞分化的影响因胶原蛋白表型而异。
