Huang B, Che D, Zhang W
Department of Pathology, Tongji Medical University, Wuhan.
J Tongji Med Univ. 1997;17(4):209-12. doi: 10.1007/BF02895621.
The effects of the medium conditioned by endothelial cells under hypoxic condition on the phenotype modulation of pulmonary artery smooth muscle cells (PASMCs) were investigated by using cell culture and morphometrical analysis. The results showed that the number of diploid cells and alpha-sm-actin of PASMCs in the hypoxic endothelial cell conditioned medium group (HECCM) was lower and the myofibrilles were even less than that in normal endothelial cell conditioned medium group (NECCM). On the contrary, the volume density of rough endoplasmic reticulum (RER) and mitochondria of PASMCs in the HECCM group was higher than that in the NECCM group, i.e. altering from a contractile type to a synthetic type. However, under direct hypoxic condition for 24 h, the phenotype of cultured PASMCs remained unchanged, i.e. retained contractile type. It suggests that the HECCM can induce phenotype modulation of PASMCs, may be mediated by the pulmonary artery endothelial cells (PAECs) which can secrete some cytokins affecting the PASMCs.
采用细胞培养和形态计量分析方法，研究了缺氧条件下内皮细胞条件培养基对肺动脉平滑肌细胞（PASMCs）表型调节的影响。结果显示，缺氧内皮细胞条件培养基组（HECCM）中PASMCs的二倍体细胞数量和α-平滑肌肌动蛋白含量低于正常内皮细胞条件培养基组（NECCM），肌原纤维也更少。相反，HECCM组中PASMCs的粗面内质网（RER）和线粒体的体积密度高于NECCM组，即从收缩型转变为合成型。然而，在直接缺氧条件下24小时，培养的PASMCs表型保持不变，即仍为收缩型。这表明HECCM可诱导PASMCs的表型调节，可能是由肺动脉内皮细胞（PAECs）介导的，PAECs可分泌一些影响PASMCs的细胞因子。
