ACS Appl Mater Interfaces. 2013 Feb;5(3):489-93. doi: 10.1021/am302554q. Epub 2013 Jan 29.
We report the concentration-dependent adsorption of serum lipoproteins onto silica nanoparticles, wherein elevated lipid levels deter complement activation. Two clinically relevant serum lipid levels - corresponding to low and borderline high levels in normal, healthy adults - were used to examine the influence of lipoprotein concentration on nanoparticle complement activation. Human serum albumin was used to study protein adsorption in the presence of lipoproteins. Preferential adsorption of high affinity lipoproteins led to greater lipid fractions in the protein corona, shielding particles from complement activation. These findings have significant implications for the design of intravenously administered carriers with biocompatible surface chemistries.
我们报告了血清脂蛋白在二氧化硅纳米颗粒上的浓度依赖性吸附，其中升高的脂质水平可阻止补体激活。使用两种临床相关的血清脂质水平（对应于正常健康成年人的低和边缘高水平）来研究脂蛋白浓度对纳米颗粒补体激活的影响。人血清白蛋白用于研究在脂蛋白存在下的蛋白质吸附。高亲和力脂蛋白的优先吸附导致蛋白质冠中的脂质分数更大，从而使颗粒免受补体激活。这些发现对设计具有生物相容性表面化学的静脉内给药载体具有重要意义。
