National Laboratory of Solid State Microstructures and Department of Physics, Nanjing University, Nanjing 210093, China.
National Laboratory of Solid State Microstructures and Department of Physics, Nanjing University, Nanjing 210093, China; Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow University, Suzhou 215006, China.
Biomaterials. 2014 Oct;35(30):8703-10. doi: 10.1016/j.biomaterials.2014.06.033. Epub 2014 Jul 5.
Understanding the role of serum protein in the process of nanoparticle delivery is of great importance in biomedicine. Here, by using dissipative particle dynamics simulations, we systematically investigate the interactions between the nanoparticle-protein corona complex and cell membranes of different types. It is found that the human serum albumin (HSA) will just adsorb onto charged (especially for positively charged) and hydrophobic nanoparticle surface. More importantly, we also provide specific insights into the effect of HSA adsorption on the in vivo transportation of nanoparticle (i.e., immune response and targeted cellular uptake). Our results show that the protein corona can change the interaction modes of hydrophobic nanoparticles and enhance the interaction of charged nanoparticles with macrophage cell membranes, while it may also cause the failure of insertion of hydrophobic nanoparticles and the loss of targeting specificity of charged nanoparticles with cancer cell membranes. These results can help better understand the biological significance of protein corona and may give some useful suggestions on better design of future nanoparticles in drug delivery.
了解血清蛋白在纳米颗粒递药过程中的作用在生物医药领域具有重要意义。在这里，我们通过使用耗散粒子动力学模拟，系统地研究了纳米颗粒-蛋白冠复合物与不同类型细胞膜之间的相互作用。结果发现，人血清白蛋白（HSA）只会吸附在带电荷（特别是带正电荷）和疏水的纳米颗粒表面。更重要的是，我们还提供了关于 HSA 吸附对纳米颗粒体内运输（即免疫反应和靶向细胞摄取）影响的具体见解。我们的结果表明，蛋白冠可以改变疏水性纳米颗粒的相互作用模式，并增强带电荷纳米颗粒与巨噬细胞膜的相互作用，同时也可能导致疏水性纳米颗粒的插入失败和带电荷纳米颗粒与癌细胞膜的靶向特异性丧失。这些结果有助于更好地理解蛋白冠的生物学意义，并可能为未来药物递送中更好地设计纳米颗粒提供一些有用的建议。
