Denovan E M, Wright J M
Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada.
Gene. 1990 Mar 15;87(2):279-83. doi: 10.1016/0378-1119(90)90313-g.
We have cloned and sequenced a highly reiterated EcoRI fragment of DNA from pollock (Pollachus virens). The EcoRI repeat is 200 (+/- 5) bp long, A + T-rich (65%) and exhibits a high degree of sequence conservation among representative members. It comprises 13% of the pollock nuclear DNA with a copy number of 5 x 10(5) per haploid genome. Partial digestion of pollock DNA with EcoRI or Hinf1, which cleaves within the repeat, followed by blot hybridization to a cloned repeat sequence, produced a ladder of hybridising bands indicating a tandemly arrayed organisation for the EcoRI repeat. Longer range periodicities, revealed by restriction endonuclease digestion, are superimposed on the tandem array.
我们已克隆并测序了来自绿青鳕（Pollachus virens）的一段高度重复的EcoRI DNA片段。该EcoRI重复序列长200（±5）碱基对，富含A+T（65%），并且在代表性成员之间表现出高度的序列保守性。它占绿青鳕核DNA的13%，每个单倍体基因组的拷贝数为5×10⁵。用EcoRI或Hinf1对绿青鳕DNA进行部分酶切（Hinf1在重复序列内切割），然后与克隆的重复序列进行印迹杂交，产生了一系列杂交条带，表明EcoRI重复序列呈串联排列。限制性内切酶消化揭示的更长范围的周期性叠加在串联阵列上。
