七鳃鳗中一个新的埃可RI卫星DNA家族。

A new EcoRI family of satellite DNA in lampreys.

作者信息

Boán F, Viñas A, Rodríguez J M, Sánchez L, Gómez-Márquez J

机构信息

Departamento de Bioquímica y Biología Molecular, Facultad de Biología, Universidad de Santiago de Compostela, Galicia, Spain.

出版信息

FEBS Lett. 1996 Sep 30;394(2):187-90. doi: 10.1016/0014-5793(96)00947-7.

DOI:10.1016/0014-5793(96)00947-7

PMID:8843161

Abstract

Satellite DNA sequences have been studied in several groups of organisms. However, until now this type of sequence has not been characterized in cyclostomata, an evolutionarily important class of vertebrates. In the present work, we report the molecular characterization of a new family of satellite DNA in lampreys (Petromyzon marinus). Digestion of lamprey DNA with EcoRI identified a series of very abundant AT-rich (60% A+T) repeating units, with short stretches of AT, that are multimers of 370 bp. Southern blot analysis and comparison with the satellite DNA sequences deposited in the databases indicate that this new family of satellite DNA is exclusive to lampreys. The distribution of this EcoRI satellite DNA on lamprey chromosomes was analyzed by in situ hybridization. The evolutionary origin of this satellite is briefly discussed.

摘要

卫星DNA序列已在几组生物体中得到研究。然而，直到现在，这种类型的序列在圆口纲动物中尚未得到表征，圆口纲是一类在进化上具有重要意义的脊椎动物。在本研究中，我们报告了七鳃鳗（海七鳃鳗）中一个新的卫星DNA家族的分子特征。用EcoRI消化七鳃鳗DNA，鉴定出一系列非常丰富的富含AT（60%A+T）的重复单元，带有短的AT片段，这些重复单元是370bp的多聚体。Southern印迹分析以及与数据库中存放的卫星DNA序列进行比较表明，这个新的卫星DNA家族是七鳃鳗所特有的。通过原位杂交分析了这种EcoRI卫星DNA在七鳃鳗染色体上的分布。简要讨论了这种卫星的进化起源。