Volobouev V, Vogt N, Viegas-Péquignot E, Malfoy B, Dutrillaux B
Institut Curie-CNRS UMR 147, 26, rue d Ulm, F-75231 Paris Cedex 05, France.
Chromosoma. 1995 Dec;104(4):252-9. doi: 10.1007/BF00352256.
Two tandemly repeated DNA sequences of Gerbillus nigeriae (Rodentia) (GN1 and GN2) were isolated and characterized. Both share a 36bp repeated unit, which includes a 20bp motif also found in primate alphoid and other repeated DNAs. The localization of GN1 and GN2 sequences on metaphase chromosomes of three Gerbillus species, G. nigeriae, G. aureus and G. nanus, was studied by fluorescence in situ hybridization (FISH). In the G. nigeriae and G. aureus karyotypes, which were shown to possess large amounts of heterochromatin and to have undergone multiple rearrangements during evolution, both GN1 and GN2 sequences were observed at various chromosomal sites: centromeric, telomeric and intercalary. In contrast, the karyotypically stable G. nanus, which does not possess large amounts of heterochromatin and seems to be a more ancestral species, possesses only GN1 sequences, localized in the juxtacentromeric regions.
分离并鉴定了尼日利亚沙鼠（啮齿目）的两个串联重复DNA序列（GN1和GN2）。二者共享一个36bp的重复单元，该单元包含一个在灵长类α卫星及其他重复DNA中也存在的20bp基序。通过荧光原位杂交（FISH）研究了GN1和GN2序列在三种沙鼠（尼日利亚沙鼠、金色沙鼠和侏沙鼠）中期染色体上的定位。在尼日利亚沙鼠和金色沙鼠的核型中，二者显示出含有大量异染色质且在进化过程中经历了多次重排，GN1和GN2序列在不同的染色体位点均有观察到：着丝粒、端粒和居间位置。相比之下，核型稳定的侏沙鼠不含有大量异染色质，似乎是一个更为原始的物种，仅拥有定位在近着丝粒区域的GN1序列。
