School of Life Science and Technology, Tongji University, Shanghai 200092, People's Republic of China.
Appl Biochem Biotechnol. 2013 Mar;169(5):1566-78. doi: 10.1007/s12010-012-0078-x. Epub 2013 Jan 16.
Gold nanostructures have generated significant attention in biomedical areas because of their major role in cancer photothermal therapeutics. In order to conveniently combine gold nanostructures and drugs into one nanocomposite, Au2Se/Au core-shell nanostructures with strong near-infrared-absorbing properties were synthesized using a simple method and embedded inside bovine serum albumin (BSA) nanospheres by using a spray dryer equipped with an ultrasonic atomizer followed by thermal denaturation. The nanospheres with narrow size distribution mainly ranging from 450 to 600 nm were obtained. The Au2Se/Au-loaded BSA nanospheres (1 mg) adsorbed at least 0.01 mg of water-insoluble zinc phthalocyanine (ZnPc) photosensitizer. After irradiation with a 655-nm laser (20 min), the temperature of the Au2Se/Au-loaded BSA nanospheres [200 μL, 2 mg/mL, BSA/Au2Se/Au 10:1 (w/w)] increased by over 20 °C from the initial temperature of 24.82 ± 0.15 °C, and the release of ZnPc was improved compared with a corresponding sample without irradiation. After being incubated with cancer cells (human esophageal carcinoma Eca-109), the nanospheres exhibited photothermal and photodynamic therapy with a synergistic effect upon laser irradiation. This work provides novel Au2Se/Au-loaded polymer nanospheres prepared by a high-efficiency strategy for incorporating drugs for improving the efficiency in killing cancer cells.
金纳米结构因其在癌症光热治疗中的重要作用而在生物医学领域引起了广泛关注。为了方便地将金纳米结构和药物结合为一种纳米复合材料，我们采用一种简单的方法合成了具有强近红外吸收性能的 Au2Se/Au 核壳纳米结构，并通过配备超声雾化器的喷雾干燥仪将其嵌入牛血清白蛋白（BSA）纳米球中，然后进行热变性。得到了具有窄尺寸分布的纳米球，主要分布在 450 至 600nm 之间。Au2Se/Au 负载的 BSA 纳米球（1mg）吸附了至少 0.01mg 的水溶性锌酞菁（ZnPc）光敏剂。用 655nm 激光照射（20min）后，Au2Se/Au 负载的 BSA 纳米球[200μL，2mg/mL，BSA/Au2Se/Au 10:1（w/w）]的温度从初始温度 24.82±0.15°C 升高了 20°C 以上，与未照射的相应样品相比，ZnPc 的释放得到了提高。将纳米球与癌细胞（人食管癌细胞 Eca-109）孵育后，在激光照射下表现出光热和光动力协同治疗作用。这项工作提供了一种高效策略制备的新型 Au2Se/Au 负载聚合物纳米球，用于整合药物以提高杀伤癌细胞的效率。
