School of Life Science and Technology, Tongji University, Shanghai 200092, P R China.
Nanoscale. 2010 Apr;2(4):542-7. doi: 10.1039/b9nr00323a. Epub 2010 Jan 14.
Differently colored quantum dot (QD) nanoparticles are incorporated into bovine serum albumin (BSA) nanospheres by spray-drying followed by thermal denaturization, which is a rapid, highly efficient, large scale, and low cost method. Because the spray-dryer is equipped with an ultrasonic atomizer, most of the nanospheres are no more than 550 nm in diameter and a have narrow size distribution. Ultrathin sections (70 nm) of nanospheres are first prepared using a technique which is normally applied to cell sectioning. The section images show that the QD-BSA nanospheres are solid, and that the QDs are successfully dispersed inside the BSA nanospheres. The nanospheres emit bright fluorescence, and their fluorescence stabilities are not obviously changed compared with that of the QDs. This work provides a novel and simple method for preparing nanoscale spheres encapsulating differently colored QDs. We also present an ultrathin sectioning method for investigating the interior details of nanomaterials.
通过喷雾干燥和热变性，将不同颜色的量子点（QD）纳米粒子掺入牛血清白蛋白（BSA）纳米球中，这是一种快速、高效、大规模和低成本的方法。由于喷雾干燥器配备了超声波雾化器，因此大多数纳米球的直径不超过 550nm，并且具有较窄的粒径分布。首先使用通常应用于细胞切片的技术制备纳米球的超薄切片（70nm）。切片图像表明，QD-BSA 纳米球是实心的，并且 QD 成功地分散在 BSA 纳米球内部。纳米球发出明亮的荧光，与 QD 相比，其荧光稳定性没有明显变化。这项工作为制备包封不同颜色 QD 的纳米级球提供了一种新颖而简单的方法。我们还提出了一种超薄切片方法，用于研究纳米材料的内部细节。
