Expression of fluorescent cyclotides using protein trans-splicing for easy monitoring of cyclotide-protein interactions.
作者信息
Jagadish Krishnappa, Borra Radhika, Lacey Vanessa, Majumder Subhabrata, Shekhtman Alexander, Wang Lei, Camarero Julio A
机构信息
Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90033, USA.
出版信息
Angew Chem Int Ed Engl. 2013 Mar 11;52(11):3126-31. doi: 10.1002/anie.201209219. Epub 2013 Jan 15.
Abstract
摘要
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本文引用的文献
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Biological synthesis of circular polypeptides.环状多肽的生物合成。
J Biol Chem. 2012 Aug 3;287(32):27026-32. doi: 10.1074/jbc.R111.305508. Epub 2012 Jun 14.
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In-cell fluorescence activation and labeling of proteins mediated by FRET-quenched split inteins.通过 FRET 猝灭分裂整合酶对细胞内蛋白质进行荧光激活和标记。
J Am Chem Soc. 2012 Apr 11;134(14):6344-53. doi: 10.1021/ja300209u. Epub 2012 Mar 28.
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Cyclotides, a novel ultrastable polypeptide scaffold for drug discovery.环肽，一种用于药物发现的新型超稳定多肽支架。
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Cellular uptake of cyclotide MCoTI-I follows multiple endocytic pathways.环肽 MCoTI-I 的细胞摄取遵循多种内吞途径。
J Control Release. 2011 Oct 30;155(2):134-43. doi: 10.1016/j.jconrel.2011.08.030. Epub 2011 Aug 30.
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Identification and characterization of a new family of cell-penetrating peptides: cyclic cell-penetrating peptides.鉴定和表征一类新的细胞穿透肽家族：环状细胞穿透肽。
J Biol Chem. 2011 Oct 21;286(42):36932-43. doi: 10.1074/jbc.M111.264424. Epub 2011 Aug 26.
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Evolution of cyclic peptide protease inhibitors.环状肽蛋白酶抑制剂的进化。
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Kinetic control of one-pot trans-splicing reactions by using a wild-type and designed split intein.利用野生型和设计的分裂内含肽对一锅法反式剪接反应进行动力学控制。
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Discovery and characterization of novel cyclotides originated from chimeric precursors consisting of albumin-1 chain a and cyclotide domains in the Fabaceae family.发现并鉴定了新型环肽，它们源自豆科植物中由白蛋白-1 链 a 和环肽结构域组成的嵌合前体。
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9
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Biosynthesis of a fluorescent protein with extreme pseudo-Stokes shift by introducing a genetically encoded non-natural amino acid outside the fluorophore.通过在荧光团外引入遗传编码的非天然氨基酸来合成具有极端赝斯托克斯位移的荧光蛋白。
J Am Chem Soc. 2011 Mar 23;133(11):3708-11. doi: 10.1021/ja1099787. Epub 2011 Feb 22.
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