Kang Youngran, Jung Woon Yong, Lee Hyunjoo, Lee Eunjung, Kim Aeree, Kim Baek-Hui
Department of Pathology, Korea University Guro Hospital, Korea University College of Medicine, Seoul, Korea.
Korean J Pathol. 2012 Dec;46(6):523-31. doi: 10.4132/KoreanJPathol.2012.46.6.523. Epub 2012 Dec 26.
Sirtuin 1 (SIRT1) and deleted in breast cancer 1 (DBC1) are known as tumor suppressor or promoter genes. This may be due to their diverse functions and interaction with other proteins. Gastric adenocarcinoma is one of the most common malignancies, but little is known about its carcinogenesis. Therefore, we investigated the association of immunohistochemical expression of SIRT1, DBC1, p53, and β-catenin and their variable clinicopathological characteristics.
We obtained samples from 452 patients who underwent gastrectomy. Tissue microarray blocks were constructed and immonohistochemical staining was performed.
Expression of DBC1 and SIRT1 was associated with lower histologic grade, intestinal type of Lauren classification, and lower pT (p<0.001) and pN stage (DBC1, p=0.002; SIRT1, p<0.001). Association between absence of lymphatic invasion, and SIRT1 (p=0.001) and DBC1 (p=0.004) was observed. Cytoplasmic β-catenin expression was associated with lower histologic grade, pT, pN, tumor-node-metastasis (TNM) stage, DBC1 (p<0.001), and SIRT1 (p=0.001). Expression of SIRT1 and DBC1 was not associated with p53 (p=0.063 and p=0.060). DBC1 was an independent good prognostic factor in multivariate analysis (p=0.012).
SIRC1 and DBC1 can be considered to be good prognostic factors in gastric adenocarcinoma.
沉默调节蛋白1（SIRT1）和乳腺癌缺失基因1（DBC1）被认为是肿瘤抑制基因或促进基因。这可能归因于它们的多种功能以及与其他蛋白质的相互作用。胃腺癌是最常见的恶性肿瘤之一，但其致癌机制尚不清楚。因此，我们研究了SIRT1、DBC1、p53和β-连环蛋白的免疫组化表达及其不同的临床病理特征之间的关联。
我们从452例行胃切除术的患者中获取样本。构建组织芯片块并进行免疫组化染色。
DBC1和SIRT1的表达与较低的组织学分级、劳伦分类的肠型以及较低的pT（p<0.001）和pN分期（DBC1，p=0.002；SIRT1，p<0.001）相关。观察到无淋巴侵犯与SIRT1（p=0.001）和DBC1（p=0.004）之间存在关联。细胞质β-连环蛋白表达与较低的组织学分级、pT、pN、肿瘤-淋巴结-转移（TNM）分期、DBC1（p<0.001）和SIRT1（p=0.001）相关。SIRT1和DBC1的表达与p53无关（p=0.063和p=0.060）。在多因素分析中，DBC1是一个独立的良好预后因素（p=0.012）。
SIRC1和DBC1可被认为是胃腺癌的良好预后因素。
