Özcan Önder, Belli Ahmet Korkut, Sakallı Çetin Esin, Kara Murat, Çelik Özgür İlhan, Kaplan Mehmet, Kayılıoğlu Selami Ilgaz, Dönmez Cem, Polat Murat
Department of General Surgery, Muğla Sıtkı Koçman University School of Medicine, Muğla, Turkey.
Department of Medical Biology, Muğla Sıtkı Koçman University School of Medicine, Muğla, Turkey.
Turk J Gastroenterol. 2019 Apr;30(4):326-330. doi: 10.5152/tjg.2019.18550.
BACKGROUND/AIMS: SIRT1 gene overexpression is reportedly associated with cancer development, via the triggering of DNA repair impairment, and cell proliferation. The study aimed to investigate SIRT1 expression in patients with gastric cancer and its correlations with the clinical and pathological characteristics of the disease.
All patients (64 patients) who underwent gastric biopsy and were diagnosed with gastric adenocarcinoma and signet ring cell carcinoma between January 2011 and December 2013 were enrolled in the study, and patients with benign gastric biopsy were enrolled in the control group (34 patients). The previously prepared gastric tissues were collected from the pathology department, and SIRT1 gene expressions were evaluated in the gastric tissues of all study patients. Patients were subclassified according to their demographic, clinical, and pathologic features, and the patient and control groups were compared.
Sixty-four patients were included in the study (25 females and 39 males). The mean age of the patients was 66±1 (range: 33-88) years. The SIRT1 gene 2' Average delta cycle threshold (CT) value was 0.102 in the control group, whereas it was 0.292 in the patients with gastric cancer (relative risk: 2.86; p=0.014). The SIRT1 gene was upregulated in all tumor stage subgroups except stage I, female patients, young patients (<45 years), and corpus and cardia tumor subgroups compared to the control group.
SIRT1 gene overexpression is associated with gastric adenocarcinoma, and it can be argued that SIRT1 gene upregulation is associated with unfavorable gastric adenocarcinoma prognosis.
背景/目的：据报道，SIRT1基因的过表达与癌症发展相关，其通过引发DNA修复损伤和细胞增殖来实现。本研究旨在调查胃癌患者中SIRT1的表达情况及其与该疾病临床和病理特征的相关性。
纳入2011年1月至2013年12月间接受胃活检并被诊断为胃腺癌和印戒细胞癌的所有患者（64例），并将胃活检为良性的患者纳入对照组（34例）。从病理科收集先前制备的胃组织，并评估所有研究患者胃组织中的SIRT1基因表达。根据患者的人口统计学、临床和病理特征对患者进行亚分类，并比较患者组和对照组。
本研究共纳入64例患者（25例女性和39例男性）。患者的平均年龄为66±1岁（范围：33 - 88岁）。对照组中SIRT1基因2'平均Δ循环阈值（CT）值为0.102，而胃癌患者中该值为0.292（相对风险：2.86；p = 0.014）。与对照组相比，除I期、女性患者、年轻患者（<45岁）以及胃体和贲门肿瘤亚组外，所有肿瘤分期亚组中的SIRT1基因均上调。
SIRT1基因过表达与胃腺癌相关，可以认为SIRT1基因上调与胃腺癌预后不良相关。
