Cui Chang-Zheng, Feng Tian-Cai, Yu Ya-Qi, Dong Fei, Yang Xin-Mei, Feng Yao-Yu, Liu Yong-Di, Lin Han-Ping
State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.
Huan Jing Ke Xue. 2012 Nov;33(11):4062-8.
Anthracene, among the 16 US EPA polycyclic aromatic hydrocarbons (PAHs), is a typical low molecular weight environmental contaminant, which gains concern on its biodegradation under hypersaline condition. In this study, an anthracene-degrading bacterial strain was isolated from highly saline petroleum-contaminated soil. Based on its physiological, biochemical characteristics and 16S rDNA sequence analysis, the bacteria was preliminary identified and named as Martelella sp. AD-3. The strain was able to utilize anthracene as sole carbon source for growth and the degradation occurred under broad salinities (0.1% to 10%) and varying pHs (6.0 to 10.0). The optimized degradation conditions were initial concentration 25 mg x L(-1), culture temperature 30 degrees C, pH 9.0 and salinity 3%. And 94.6% of anthracene was degraded by strain AD-3 under the optimal conditions within 6 days. Degenerate primers design was performed with a reported dioxygenase alpha subunit homologous gene. A length of 307 bp fragment of the partial dioxygenase gene sequences (GenBank accession: JF823991.1) was amplified by nested PCR. The clones amino acid sequence from strain AD-3 showed 95% identity to that of the partial naphthalene dioxygenase large-subunit from Marinobacter sp. NCE312 (AF295033). The results lay a foundation for the further study of molecular mechanism involved in the PAHs biodegradation by strain AD-3.
在16种美国环保署多环芳烃（PAHs）中，蒽是一种典型的低分子量环境污染物，其在高盐条件下的生物降解受到关注。在本研究中，从高盐石油污染土壤中分离出一株蒽降解细菌菌株。基于其生理生化特性和16S rDNA序列分析，该细菌被初步鉴定并命名为Martelella sp. AD - 3。该菌株能够利用蒽作为唯一碳源进行生长，并且在较宽的盐度范围（0.1%至10%）和不同的pH值（6.0至10.0）下都能发生降解。优化后的降解条件为初始浓度25 mg x L(-1)、培养温度30℃、pH 9.0和盐度3%。在最佳条件下，菌株AD - 3在6天内可降解94.6%的蒽。利用已报道的双加氧酶α亚基同源基因设计简并引物。通过巢式PCR扩增出一段长度为307 bp的部分双加氧酶基因序列片段（GenBank登录号：JF823991.1）。菌株AD - 3的克隆氨基酸序列与Marinobacter sp. NCE312（AF295033）的部分萘双加氧酶大亚基的氨基酸序列同一性为95%。这些结果为进一步研究菌株AD - 3参与多环芳烃生物降解的分子机制奠定了基础。
