Xu Li, Gao Zhen, Luo Mu, Cheng Yanjun, Jin Jinghua
Environmental Protection Research Institute of Light Industry, Beijing 100089, China.
Wei Sheng Wu Xue Bao. 2011 Mar;51(3):313-9.
The aim of this study was to isolate high-molecular-weight polycyclic aromatic hydrocarbons (HMW-PAHs) degrading bacterial strains, and to study their degradation potential.
We used sublimation method to enrich and isolate the degrading bacteria from coking plant samples. Morphological properties, the sequence homology of 16S rRNA and gyrb genes were used to identify the isolated strains. GC-MS was used to analyze the degradation potential against some HMW-PAHs.
An HMW-PAHs degrading bacterium,HBS1, was obtained. HBS1 could use several HMW-PAHs such as pyrene, benzoanthracene, benzopyrene, chyrsene, indeno[ 1,2,3-cd] pyrene, benzo[g,h,i]perylene and fluoranthene as sole carbon source for growth. HBS1 was identified as Gordonia sp., based on the high sequence similarities (more than 99%) of both 16S rRNA gene and gyrb gene to those of Gordonia amicalis. When the initial concentration of pyrene was 50 mg/L, HBS1 could consume 97% of the pyrene in 17 days. One fragment of the dioxygenase gene was obtained by PCR with size about 300 bp, which was closest to the counterpart from Mycobacterium sp. with 93.8% similarity.
We isolated a strain HBS1 from seriously PAHs-polluted soils and identified as a Gordonia sp. The isolate had the degradation potential of high-molecular weight polycyclic aromatic hydrocarbons.
本研究旨在分离降解高分子量多环芳烃（HMW-PAHs）的细菌菌株，并研究其降解潜力。
我们采用升华法从焦化厂样品中富集和分离降解细菌。利用形态学特性、16S rRNA和gyrb基因的序列同源性对分离出的菌株进行鉴定。采用气相色谱-质谱联用（GC-MS）分析其对某些HMW-PAHs的降解潜力。
获得了一株降解HMW-PAHs的细菌HBS1。HBS1能够利用芘、苯并蒽、苯并芘、屈、茚并[1,2,3-cd]芘、苯并[g,h,i]苝和荧蒽等多种HMW-PAHs作为唯一碳源进行生长。基于16S rRNA基因和gyrb基因与友好戈登氏菌的序列高度相似性（超过99%），HBS1被鉴定为戈登氏菌属。当芘的初始浓度为50 mg/L时，HBS1在17天内可消耗97%的芘。通过PCR获得了一个大小约为300 bp的双加氧酶基因片段，其与分枝杆菌属的对应片段相似度最高，为93.8%。
我们从多环芳烃污染严重的土壤中分离出一株菌株HBS1，鉴定为戈登氏菌属。该菌株具有降解高分子量多环芳烃的潜力。
