Leifer Cynthia A, Rose William A, Botelho Fernando
Department of Microbiology and Immunology, Cornell University, Ithaca, NY, USA.
J Immunoassay Immunochem. 2013;34(1):1-15. doi: 10.1080/15321819.2012.666222.
Understanding the mechanistic basis of receptor activation and regulation can offer therapeutic targets for disease treatment. Evidence is emerging for a role of the normally foreign responsive Toll-like receptors (TLRs) in the development of autoimmunity through response to self-patterns. Regulatory mechanisms governing this class of receptors are poorly understood, and failures within this system likely contribute to development of autoimmunity. In this article, we review biochemical assays used to study one of the self-pattern responsive TLRs, TLR9, and suggest that these studies are critical for development of new targets for autoimmune therapies.
了解受体激活和调节的机制基础可为疾病治疗提供治疗靶点。越来越多的证据表明，正常情况下对外来物质有反应的Toll样受体（TLR）通过对自身模式的反应在自身免疫发展中发挥作用。人们对这类受体的调节机制了解甚少，而该系统内的功能失调可能会导致自身免疫的发展。在本文中，我们综述了用于研究对自身模式有反应的TLR之一TLR9的生化分析方法，并表明这些研究对于开发自身免疫疗法的新靶点至关重要。
